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固定对肠道活检标本石蜡切片中浆细胞免疫过氧化物酶染色的影响。

The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.

作者信息

Bosman F T, Lindeman J, Kuiper G, van der Wal A, Kreunig J

出版信息

Histochemistry. 1977 Jul 18;53(1):57-62. doi: 10.1007/BF00511210.

Abstract

The influence of different fixation methods on the results of immunoperoxydase staining of immunoglobulin and gastrin producing cells in gastric and duodenal mucosa was investigated. An indirect method was used on paraffin sections. It appeared that that fixatives containing sublimate gave the most consistent results, a sublimate-formaldehyde mixture being the best.

摘要

研究了不同固定方法对胃和十二指肠黏膜中免疫球蛋白及胃泌素产生细胞免疫过氧化物酶染色结果的影响。在石蜡切片上采用间接法。结果显示,含升汞的固定剂效果最为一致,其中升汞-甲醛混合固定剂最佳。

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