Curran R C, Gregory J
J Clin Pathol. 1980 Nov;33(11):1047-57. doi: 10.1136/jcp.33.11.1047.
A number of fixatives were tested to determine their suitability for use with the unlabelled antibody peroxidase-antiperoxidase (PAP) method for demonstrating immunoglobulin in paraffin sections of tonsil and trephine samples of bone marrow. It was found that tonsil fixed in 'isotonic' solutions of formaldehyde reacted with the PAP method only after the sections had been trypsinised. Several other fixatives, including Bouin's fluid, Carnoy's fluid, and solutions containing mercuric chloride, gave tissues which reacted without trypsinisation of sections, and particularly good results were obtained with formol saline to which acetic acid (2-10%) had been added. A combination of acetic acid (10%)-formol saline and formol sublimate also gave excellent results with bone marrow. The influence on the PAP method of a number of steps in the processing of tissues and sections was also examined.
测试了多种固定剂，以确定它们是否适用于未标记抗体过氧化物酶-抗过氧化物酶（PAP）法，用于在扁桃体石蜡切片和骨髓环钻样本中显示免疫球蛋白。结果发现，用甲醛“等渗”溶液固定的扁桃体，只有在切片经胰蛋白酶处理后，才会与PAP法发生反应。其他几种固定剂，包括Bouin氏液、Carnoy氏液以及含氯化汞的溶液，所处理的组织在切片未经胰蛋白酶处理的情况下就能发生反应，尤其向其中加入了乙酸（2-10%）的甲醛生理盐水效果特别好。乙酸（10%）-甲醛生理盐水和升汞甲醛的组合用于骨髓时也能得到极佳的效果。还研究了组织和切片处理过程中多个步骤对PAP法的影响。
