School of Health Sciences, 12390Wuhan University, China.
Nell Hodgson Woodruff School of Nursing, 1371Emory University, Atlanta, GA, USA.
Biol Res Nurs. 2021 Jul;23(3):382-393. doi: 10.1177/1099800420975129. Epub 2020 Dec 3.
The colonization characteristics of infant gut microbiota are influenced by many factors at various stages, but few studies have explored the longitudinal effects of environmental tobacco smoke exposure and quantitative breastfeeding duration on young children' gut microbiota. We explored the effects of smoke exposure and breastfeeding duration on gut microbiota by following 37 maternal and children pairs in China for 2 years. We collected the demographic information, frequency of smoke exposure, breastfeeding duration, and fecal samples (mothers in the late pregnancy and infants at 6, 12, and 24 months), and analyzed the microbiota results using the V3-V4 gene sequence of 16S rRNA. The diversity of gut microbiota in children was the highest at 24 months and most similar to that in mothers. Breastfeeding duration was positively correlated with and negatively correlated with The α diversity of microbiota and the relative abundance of was higher in the non-smoke exposed group. The higher the smoke exposure, the higher the relative abundance of Prolonged breastfeeding and reduced smoke exposure are beneficial to the diversity and composition of gut microbiota in young children.
婴幼儿肠道微生物群的定植特征受到各阶段多种因素的影响，但很少有研究探讨环境烟草烟雾暴露和母乳喂养时间定量对幼儿肠道微生物群的纵向影响。我们通过对中国的 37 对母婴进行为期 2 年的随访，探讨了烟雾暴露和母乳喂养时间对肠道微生物群的影响。我们收集了人口统计学信息、烟雾暴露频率、母乳喂养时间以及粪便样本（母亲在妊娠晚期和婴儿在 6、12 和 24 个月时），并使用 16S rRNA 的 V3-V4 基因序列分析了微生物组的结果。儿童的肠道微生物群多样性在 24 个月时最高，与母亲的最相似。母乳喂养时间与 呈正相关，与 呈负相关。非吸烟组的微生物群 α 多样性和 的相对丰度更高。烟雾暴露越高， 的相对丰度越高。延长母乳喂养时间和减少烟雾暴露有利于幼儿肠道微生物群的多样性和组成。
