Huang Zhaohe, Liu Yan, Liang Limei, Liu Wenjing, Sooranna Suren R, Mo Jianjiao, Liu Li, Li Zhile, Li Kela, Guo Jun
Department of Cardiology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China.
Department of Cardiology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, P.R. China.
Exp Ther Med. 2019 Sep;18(3):2346-2352. doi: 10.3892/etm.2019.7805. Epub 2019 Jul 24.
The present study attempted to determine the correlation of the degree of coronary artery stenosis and Tolllike receptor 2/4 (TLR2/4) levels in Chinese Zhuang patients with coronary heart disease (CHD). A total of 466 Chinese patients from the Zhuang Ethnic population diagnosed with CHD at the Department of Cardiology the Affiliated Hospital of Youjiang Medical University between January 2016 and August 2017, together with 102 control patients, were recruited for the present study. The patients with CHD were divided into three groups depending on the number of diseased arteries. The patients with CHD were also classified according to their Gensini scores. Blood liver and renal function parameters, as well as blood sugar and lipid levels were measured. ELISA was used for TLR2/4 measurements. There were no significant differences with gender, age and body mass index between the CHD and control groups. The levels of TLR2/4 in the peripheral blood of the control and CHD groups were 2.34±0.85/5.08±2.41 and 5.22±3.16/9.33±4.92 ng/ml, respectively, and the differences were significant (P<0.001). Analysis of the three subgroups of vessel disease indicated that the expression of TLR2/4 was progressively higher with the increase in the number of affected vessels (P<0.01). There were also significant differences between the mild, moderate and severe stenosis groups (P<0.01). A positive linear correlation between TLR2/4 and the Gensini coronary artery score was identified (r=0.508 and 0.346, respectively; P<0.0001). In conclusion, the present study determined a positive correlation between the degree of coronary artery stenosis and the expression level of TLR2/4 in the serum of Chinese Zhuang patients with CHD. Serum TLR2/4 may be used to predict the severity of CHD.
本研究旨在确定中国壮族冠心病（CHD）患者冠状动脉狭窄程度与Toll样受体2/4（TLR2/4）水平之间的相关性。2016年1月至2017年8月期间，在右江民族医学院附属医院心内科诊断为CHD的466名中国壮族患者以及102名对照患者被纳入本研究。CHD患者根据病变动脉数量分为三组。CHD患者还根据其Gensini评分进行分类。测量了血液肝肾功能参数以及血糖和血脂水平。采用酶联免疫吸附测定法（ELISA）检测TLR2/4。CHD组和对照组在性别、年龄和体重指数方面无显著差异。对照组和CHD组外周血中TLR2/4水平分别为2.34±0.85/5.08±2.41和5.22±3.16/9.33±4.92 ng/ml，差异有统计学意义（P<0.001）。血管疾病三个亚组的分析表明，TLR2/4的表达随着受累血管数量的增加而逐渐升高（P<0.01）。轻度、中度和重度狭窄组之间也有显著差异（P<0.01）。确定TLR2/4与Gensini冠状动脉评分之间存在正线性相关性（r分别为0.508和0.346；P<0.0001）。总之，本研究确定中国壮族CHD患者冠状动脉狭窄程度与血清中TLR2/4表达水平之间存在正相关。血清TLR2/4可用于预测CHD的严重程度。
