Stolbova A V
Genetika. 1987 Dec;23(12):2128-37.
Cloning and segregation analysis of polyauxotrophic (PA) progeny, as well as the study of diploid segregants, revealed an unusual state of the diploid genome in these strains. Analysis of linkage markers of chromosome III in all crosses may be expected to illuminate relationships between the homologous chromosomes. All PA strains were assigned to two major classes. In the class of PA strains with recombinant chromosome III, the effect of approachment of third linkage markers was noted. In other strains, transposition of genetic marker metA1 to chromosome III and its linkage to leu2 were discovered. The present study has demonstrated that the unusual state of the diploid genome of PA strains induces multiple nonspecific alterations in chromosome relationship and structure. It is likely that these alterations are the consequence of disturbance in mitotic apparatus of cell.
多营养缺陷型（PA）后代的克隆与分离分析，以及二倍体分离株的研究，揭示了这些菌株中二倍体基因组的异常状态。对所有杂交中III号染色体的连锁标记进行分析，有望阐明同源染色体之间的关系。所有PA菌株被分为两大类。在具有重组III号染色体的PA菌株类别中，注意到第三个连锁标记接近的效应。在其他菌株中，发现遗传标记metA1转座到III号染色体并与leu2连锁。本研究表明，PA菌株二倍体基因组的异常状态会诱导染色体关系和结构的多种非特异性改变。这些改变很可能是细胞有丝分裂装置紊乱的结果。
