Department of Prosthodontics, School of Dentistry, Showa University.
Department of Conservative Dentistry, Division of Biomaterials and Engineering, School of Dentistry, Showa University.
Dent Mater J. 2021 Mar 31;40(2):472-480. doi: 10.4012/dmj.2019-435. Epub 2020 Dec 2.
We evaluated the biological effects of implant abutments made from ceria-stabilized zirconia/alumina nanocomposite (Ce-TZP/AlO) with surface roughness variations using human gingival fibroblasts (HGF-1) in the transmucosal region. Two types of titanium (Ti) and Ce-TZP/AlO disks with different surface roughness profiles were prepared (Ra0.9 and Ra0.02). Surface properties were evaluated using SEM, EDX, and wettability analysis. Biological parameters including cell adhesion, proliferation and morphology, collagen deposition, and inflammatory cytokine expression were evaluated for each disk. Surface morphology analysis of Ce-TZP/AlO and Ti elucidated the uniform linear structures of Ra0.9 and the smooth and flat structures of Ra0.02. Cell morphology showed spindle-shaped and large, circular forms, respectively. Cell adhesion and proliferation and collagen deposition were significantly increased on Ce-TZP/AlO Ra0.02 disk compared with the others, with no significant differences in cytokine expression among all the disks. The reduced surface roughness of Ce-TZP/AlO was advantageous for promoting biological effects in the transmucosal region.
我们评估了具有粘膜下区域内表面粗糙度变化的氧化铈稳定氧化锆/氧化铝纳米复合材料（Ce-TZP/AlO）种植体基台的生物效应。制备了两种不同表面粗糙度轮廓的钛（Ti）和 Ce-TZP/AlO 盘（Ra0.9 和 Ra0.02）。使用 SEM、EDX 和润湿性分析评估表面特性。评估了每个磁盘的细胞粘附、增殖和形态、胶原蛋白沉积和炎症细胞因子表达等生物参数。Ce-TZP/AlO 和 Ti 的表面形貌分析显示 Ra0.9 的均匀线性结构和 Ra0.02 的光滑和平坦结构。细胞形态分别呈梭形和大圆形。与其他磁盘相比，Ce-TZP/AlO Ra0.02 磁盘上的细胞粘附、增殖和胶原蛋白沉积显著增加，所有磁盘的细胞因子表达无显著差异。Ce-TZP/AlO 的表面粗糙度降低有利于促进粘膜下区域的生物效应。
