舌和舌下神经形态学后舌内霍乱毒素 B 链霉素注射。
Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection.
机构信息
Department of Biomedical Sciences, University of Missouri, Columbia, Missouri, USA.
Department of Otolaryngology-Head and Neck Surgery, University of Missouri, Columbia, Missouri, USA.
出版信息
Muscle Nerve. 2021 Mar;63(3):413-420. doi: 10.1002/mus.27131. Epub 2020 Dec 15.
INTRODUCTION
We recently developed an inducible model of dysphagia using intralingual injection of cholera toxin B conjugated to saporin (CTB-SAP) to cause death of hypoglossal neurons. In this study we aimed to evaluate tongue morphology and ultrastructural changes in hypoglossal neurons and nerve fibers in this model.
METHODS
Tissues were collected from 20 rats (10 control and 10 CTB-SAP animals) on day 9 post-injection. Tongues were weighed, measured, and analyzed for microscopic changes using laminin immunohistochemistry. Hypoglossal neurons and axons were examined using transmission electron microscopy.
RESULTS
The cross-sectional area of myofibers in the posterior genioglossus was decreased in CTB-SAP-injected rats. Degenerative changes were observed in both the cell bodies and distal axons of hypoglossal neurons.
DISCUSSION
Preliminary results indicate this model may have translational application to a variety of neurodegenerative diseases resulting in tongue dysfunction and associated dysphagia.
简介
我们最近开发了一种使用霍乱毒素 B 与相思豆毒素（CTB-SAP）连接物进行舌内注射的吞咽困难诱导模型，以导致舌下神经元死亡。在这项研究中，我们旨在评估该模型中舌下神经元和神经纤维的舌形态和超微结构变化。
方法
在注射后第 9 天，从 20 只大鼠（10 只对照和 10 只 CTB-SAP 动物）中收集组织。测量舌的重量、长度，并使用层粘连蛋白免疫组织化学分析微观变化。使用透射电子显微镜检查舌下神经元和轴突。
结果
注射 CTB-SAP 的大鼠的后颏舌肌肌纤维的横截面积减小。舌下神经元的胞体和远侧轴突均观察到退行性变化。
讨论
初步结果表明，该模型可能具有向多种导致舌功能障碍和相关吞咽困难的神经退行性疾病转化的应用潜力。
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