Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Key Laboratory of Microbial Resources Exploition and Application, Gansu Province, Lanzhou 730000, PR China.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004594. Epub 2020 Dec 3.
A bacterial strain, designated Y40, was isolated from sandy soil sampled on the Qinghai-Tibet Plateau. A polyphasic study confirmed the affiliation of the strain with the genus . Strain Y40 was found to be an aerobic, Gram-stain-positive, motile and rod-shaped bacterium. The strain grew at 10-42 °C, pH 6-9 and with 0-2 % (w/v) NaCl. The diagnostic amino acid was -diaminopimeilic acid. MK7 was predominant menaquinone, and iso-C, iso-C ω10 and anteiso-C were the major fatty acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The DNA G+C content was 40.6 mol%. Based on he results of 16S rRNA gene sequence analysis, strain Y40 was phylogenetically closely related to JJ-247 and CV53, with similarities of 98.0 and 97.7 %, respectively. The average nucleotide identity (ANIb) values between strain Y40 and JJ-247 and CV53 were 69.9 and 70.0 %, respectively. Based on the morphological, physiological, and chemotaxonomic data, it is proposed that strain Y40 (=CICC 24459=JCM 32794) should be classified into the genus as sp. nov.
一株细菌菌株，命名为 Y40，从青藏高原采集的沙土中分离得到。多相研究证实该菌株属于 属。菌株 Y40 是需氧、革兰氏阳性、能动和杆状细菌。该菌株在 10-42°C、pH 值 6-9 和 0-2%（w/v）NaCl 条件下生长。其特征性氨基酸为 -二氨基庚二酸。主要的类异戊二烯醌为 MK7，主要脂肪酸为 iso-C、iso-C ω10 和 anteiso-C。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的脂。DNA G+C 含量为 40.6 mol%。基于 16S rRNA 基因序列分析的结果，菌株 Y40 与 JJ-247 和 CV53 亲缘关系最密切，相似性分别为 98.0%和 97.7%。菌株 Y40 与 JJ-247 和 CV53 的平均核苷酸同一性（ANIb）值分别为 69.9%和 70.0%。基于形态学、生理学和化学分类学数据，建议将菌株 Y40（=CICC 24459=JCM 32794）归类为 属的一个新种。
