Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2020 Jan;70(1):631-635. doi: 10.1099/ijsem.0.003806.
A Gram-stain-positive, aerobic bacterium, designated CPCC 204705, was isolated from a desert soil sample, collected from the Badain Jaran desert. Growth of strain CPCC 204705 was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705 belonged to the genus , showing the highest similarity (98.54 %) of 16S rRNA gene sequence to JCM 17534. The peptidoglycan type was A4, containing d-ornithine and d-glutamic acids as diagnostic amino acids. Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars. The major cellular fatty acids were anteiso-C, anteiso-CA, C and C. The major menaquinone was MK-9 (H) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids. The DNA-DNA hybridization value between strain CPCC 204705 and JCM 17534 was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %. The DNA G+C content of strain CPCC 204705 was 75.4 mol%. Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705 should be classified as a novel species. The name sp. nov. is proposed, with strain CPCC 204705 (=DSM 105430=KCTC 39974) as the type strain.
一株革兰氏阳性、需氧细菌，被命名为 CPCC 204705，从巴丹吉林沙漠的沙漠土壤样本中分离得到。CPCC 204705 菌株的生长条件为 pH6.0-8.0 和 15-37°C，最适生长条件为 28°C 和 pH7.0。基于 16S rRNA 基因序列的系统发育分析表明，CPCC 204705 菌株属于 属，与 JCM 17534 的 16S rRNA 基因序列相似度最高（98.54%）。肽聚糖类型为 A4，含有 d-鸟氨酸和 d-谷氨酸作为诊断性氨基酸。在全细胞水解物中检测到鼠李糖和半乳糖作为诊断性糖。主要的细胞脂肪酸为 anteiso-C、anteiso-CA、C 和 C。主要的menaquinone 为 MK-9（H），极性脂类系统包含双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇甘露糖苷、一种未鉴定的脂类、一种未鉴定的氨基脂类和两种未鉴定的氨基磷脂类。CPCC 204705 菌株与 JCM 17534 之间的 DNA-DNA 杂交值为 7.1±0.4 %，这两个菌株之间的平均核苷酸同一性值为 79.8 %。CPCC 204705 菌株的 DNA G+C 含量为 75.4 mol%。基于生理实验、化学分类学数据、系统发育分析和 DNA-DNA 杂交值的结果，CPCC 204705 菌株应归类为一个新的 种。建议使用新名称 sp. nov.，CPCC 204705 菌株（=DSM 105430=KCTC 39974）作为模式菌株。
