Innovation Center, Amano Enzyme Inc., 1-6 Technoplaza, Kakamigahara, Gifu 509-0109, Japan.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005253.
A Gram-stain-variable, motile, aerobic, spore-forming, rod-shaped bacterium, designated as strain LOB 377, was isolated from soil sampled in Koka County (now Konan City), in Shiga Prefecture, Japan. To determine its taxonomic position, the bacterium was evaluated by a polyphasic approach based on genomic, phenotypic and chemotaxonomic tests. From phylogenetic analysis based on 16S rRNA gene sequences, strain LOB 377 (LC570960) was revealed to have the highest similarity to the type strain of FJAT-13985 (9.1 %). The average nucleotide identity and digital DNA-DNA hybridization values between both strains based on whole genome sequences were 84.1 and 42.8 %, respectively, which were below the recommended thresholds. The strain grew at 15-45 °C (optimum, 25-37 °C), at pH 5.5-9.5 (optimum, pH 6.5-8.5) and with 1.0-2.0 % (w/v) NaCl. Cell-wall peptidoglycan of the strain contained -diaminopimelic acid. The major menaquinone was menaquinone-7. Predominant fatty acids were iso-C, anteiso-C, iso-C and C ω11. The polar lipids were phosphatidylglycerol, unidentified phospholipid and unidentified aminophospholipid. The DNA G+C content was 40.5 mol%. According to the genomic, phenotypic and chemotaxonomic results, strain LOB 377 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is LOB 377 (=ATCC 31382=NBRC 114637=DSM 113418).
一株革兰氏染色可变、运动性、需氧、产芽孢的杆状细菌，被命名为LOB 377 菌株，从日本滋贺县（现改称近江八幡市）小和郡采集的土壤中分离得到。为了确定其分类地位，采用基于基因组、表型和化学分类学测试的多相方法对该细菌进行了评估。基于 16S rRNA 基因序列的系统发育分析表明，LOB 377 株（LC570960）与 FJAT-13985 型菌株（9.1%）的相似度最高。基于全基因组序列，两菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 84.1%和 42.8%，均低于推荐阈值。该菌株在 15-45°C（最佳温度为 25-37°C）、pH 5.5-9.5（最佳 pH 为 6.5-8.5）和 1.0-2.0%（w/v）NaCl 下生长。细胞壁肽聚糖含有 -二氨基庚二酸。主要的menaquinone 是menaquinone-7。主要脂肪酸为 iso-C、anteiso-C、iso-C 和 Cω11。极性脂为磷脂酰甘油、未鉴定的磷脂和未鉴定的氨基磷脂。DNA G+C 含量为 40.5mol%。根据基因组、表型和化学分类学结果，LOB 377 株代表了属中的一个新种，建议将其命名为 sp. nov.。模式菌株为 LOB 377（=ATCC 31382=NBRC 114637=DSM 113418）。
