Department of Anesthesiology, University Hospital Duesseldorf, Duesseldorf, Germany.
Department of Internal Medicine, Elbe Clinics Stade-Buxtehude, Stade, Germany.
PLoS One. 2020 Dec 3;15(12):e0243220. doi: 10.1371/journal.pone.0243220. eCollection 2020.
Coronary effluent collected from ischemic preconditioning (IPC) treated hearts induces myocardial protection in non-ischemic-preconditioned hearts. So far, little is known about the number of IPC cycles required for the release of cardioprotective factors into the coronary effluent to successfully induce cardioprotection. This study investigated the cardioprotective potency of effluent obtained after various IPC cycles in the rat heart. Experiments were performed on isolated hearts of male Wistar rats, mounted onto a Langendorff system and perfused with Krebs-Henseleit buffer. In a first part, effluent was taken before (Con) and after each IPC cycle (Eff 1, Eff 2, Eff 3). IPC was induced by 3 cycles of 5 min of global myocardial ischemia followed by 5 minutes of reperfusion. In a second part, hearts of male Wistar rats were randomized to four groups (each group n = 4-5) and underwent 33 min of global ischemia followed by 60 min of reperfusion. The previously obtained coronary effluent was administered for 10 minutes before ischemia as a preconditioning stimulus. Infarct size was determined at the end of reperfusion by triphenyltetrazoliumchloride (TTC) staining. Infarct size with control effluent was 54±12%. Effluent obtained after IPC confers a strong infarct size reduction independent of the number of IPC cycles (Eff 1: 27±5%; Eff 2: 35±7%; Eff 3: 35±8%, each P<0.05 vs. Con). Effluent extracted after one cycle IPC is comparably protective as after two or three cycles IPC.
从缺血预处理 (IPC) 处理的心脏中收集的冠状流出物可诱导非缺血预处理心脏的心肌保护。到目前为止，对于释放到冠状流出物中的保护因子的数量，还知之甚少，需要多少个 IPC 循环才能成功诱导心肌保护。本研究调查了在大鼠心脏中进行不同 IPC 循环后获得的流出物的心肌保护效力。实验在雄性 Wistar 大鼠的离体心脏上进行，将其安装在 Langendorff 系统上，并使用 Krebs-Henseleit 缓冲液进行灌注。在第一部分中，在每个 IPC 循环之前（Con）和之后（Eff 1、Eff 2、Eff 3）采集流出物。IPC 通过 3 个 5 分钟的全心缺血循环和 5 分钟的再灌注诱导。在第二部分，雄性 Wistar 大鼠的心脏随机分为四组（每组 n = 4-5），进行 33 分钟的全心缺血，随后进行 60 分钟的再灌注。之前获得的冠状流出物在缺血前作为预处理刺激给药 10 分钟。在再灌注结束时通过三苯基四唑氯化物（TTC）染色确定梗塞面积。用对照流出物处理的梗塞面积为 54±12%。IPC 后获得的流出物独立于 IPC 循环的数量赋予强烈的梗塞面积减少（Eff 1：27±5%；Eff 2：35±7%；Eff 3：35±8%，每个 P<0.05 与 Con 相比）。IPC 后提取的一个循环的流出物与两个或三个循环 IPC 后的流出物具有相当的保护作用。
