从废弃矿山中分离的深海盐单胞菌对水溶液中 Pb（II）和 Zn（II）的生物吸附。

Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine.

机构信息

Faculty of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-Ku, Sapporo, 060-8628, Japan.

IWRM Centre/Geology Department, School of Mines, University of Zambia, P.O. Box 32379, Lusaka, Zambia.

出版信息

Sci Rep. 2020 Dec 3;10(1):21189. doi: 10.1038/s41598-020-78187-4.

The present study investigated biosorption of Pb (II) and Zn (II) using a heavy metal tolerant bacterium Oceanobacillus profundus KBZ 3-2 isolated from a contaminated site. The effects of process parameters such as effect on bacterial growth, pH and initial lead ion concentration were studied. The results showed that the maximum removal percentage for Pb (II) was 97% at an initial concentration of 50 mg/L whereas maximum removal percentage for Zn (II) was at 54% at an initial concentration of 2 mg/L obtained at pH 6 and 30 °C. The isolated bacteria were found to sequester both Pb (II) and Zn (II) in the extracellular polymeric substance (EPS). The EPS facilitates ion exchange and metal chelation-complexation by virtue of the existence of ionizable functional groups such as carboxyl, sulfate, and phosphate present in the protein and polysaccharides. Therefore, the use of indigenous bacteria in the remediation of contaminated water is an eco-friendly way of solving anthropogenic contamination.

本研究采用从污染场地中分离出的耐重金属细菌 Oceanobacillus profundus KBZ3-2 来研究 Pb(II) 和 Zn(II) 的生物吸附。研究了过程参数的影响，如对细菌生长的影响、pH 值和初始铅离子浓度。结果表明，在初始浓度为 50mg/L 时，Pb(II) 的最大去除率为 97%，而在初始浓度为 2mg/L 时，Zn(II) 的最大去除率为 54%，pH 值为 6，温度为 30°C。分离出的细菌被发现将 Pb(II) 和 Zn(II) 都固定在细胞外聚合物质 (EPS) 中。EPS 通过存在可电离的功能基团，如羧基、硫酸根和磷酸根，在蛋白质和多糖中，促进离子交换和金属螯合-配位。因此，利用土著细菌修复受污染的水是解决人为污染的一种环保方式。