Kalita Debajit, Joshi S R
Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
Biotechnol Rep (Amst). 2017 Nov 8;16:48-57. doi: 10.1016/j.btre.2017.11.003. eCollection 2017 Dec.
Lead released from manufacturing factories, recycling plants, automobile company and landfill leachate is abundantly found in wastewater. An efficient bioremediating agent for lead removal from wastewater is expected to ease the ever increasing problem. The present study reports sp. W6 isolated from extreme habitat of hot water spring of North-East India evaluated for its Lead biosorption property. The bacterium showed capacity to resist 1.0 mM lead in both solid and liquid minimal media. Epifluorescence microscopy reveal the viability of bacterial cells under metal stress condition. ICP-MS analysis revealed 65% and 61.2% removal of lead from the Synthetic Bangladesh Ground Water medium in batch culture and column study respectively which was higher when compared to biosorption capacity of 2474, MJ7 from forest soil and PKRS11 from uranium rich soil. Exopolysaccharide released by the isolate which influenced biosorption revealed the presence of ligands assayed using microbial hydrophobicity and FTIR. The extremophilic isolate is proposed as a choice for efficient bioremediation of lead contaminated wastewater.
从制造工厂、回收厂、汽车公司和垃圾渗滤液中释放的铅在废水中大量存在。一种用于从废水中去除铅的高效生物修复剂有望缓解日益严重的问题。本研究报告了从印度东北部热水泉的极端栖息地分离出的W6菌株，对其铅生物吸附特性进行了评估。该细菌在固体和液体基本培养基中均显示出抵抗1.0 mM铅的能力。落射荧光显微镜显示了金属胁迫条件下细菌细胞的活力。电感耦合等离子体质谱分析表明，在分批培养和柱研究中，分别从合成孟加拉国地下水介质中去除了65%和61.2%的铅，与从森林土壤中分离的2474、MJ7以及从富铀土壤中分离的PKRS11的生物吸附能力相比更高。该分离物释放的影响生物吸附的胞外多糖显示了使用微生物疏水性和傅里叶变换红外光谱测定的配体的存在。该嗜极端菌被提议作为高效生物修复铅污染废水的选择。
