Hangzhou Key Laboratory for Ecosystem Protection and Restoration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
Integr Zool. 2021 May;16(3):404-419. doi: 10.1111/1749-4877.12506. Epub 2020 Dec 21.
We used mitochondrial cytochrome b and ND4 genes and 9 microsatellite loci to determine genetic diversity, population structure, evolutionary history, and migration patterns within the Reeves' butterfly lizard Leiolepis reevesii (Agamidae). Considering molecular-based phylogeographical lineages, we then performed niche equivalency and similarity tests between divergent lineages. Phylogenetic analyses based on mitochondrial DNA (mtDNA) data revealed 2 lineages (A and B) diverging ≈0.84 million years ago and, respectively, restricted to the northern and southern portions of the Wuzhishan and Yinggeling mountain ranges. Lineage B contains individuals from southern Hainan; Lineage A includes individuals from all other localities and can be further divided into 3 clusters according to microsatellite data. The null hypothesis that the 2 lineages shared identical niches was rejected in all niche equivalency tests, indicating niche shifts during genetic divergence. Similarity tests provided evidence of niche conservatism, suggesting that the 2 lineages share more characteristics of their niche spaces than randomly expected. The niche similarity and equivalency tests indicated a complex niche pattern in which both lineages share a main portion of their ecological spaces. The climatic niche of Lineage B represented a marginal and specialized fraction of the entire ecological space of the climatic niche of Lineage A, with warmer conditions. Isolation caused by orogenesis and subsequent niche divergence, together with local adaptation, may have led to genetic differentiation and further lineage sorting in L. reevesii.
我们使用线粒体细胞色素 b 和 ND4 基因以及 9 个微卫星位点来确定 Reeves' 蝴蝶蜥 Leiolepis reevesii（鬣蜥科）的遗传多样性、种群结构、进化历史和迁徙模式。考虑到基于分子的系统地理学谱系，我们随后在分歧的谱系之间进行了生态位等效性和相似性测试。基于线粒体 DNA（mtDNA）数据的系统发育分析揭示了 2 个谱系（A 和 B）在约 84 万年前分化，并分别局限于五指山和鹦哥岭山脉的北部和南部。谱系 B 包含来自海南南部的个体；谱系 A 包含来自所有其他地点的个体，并可以根据微卫星数据进一步分为 3 个聚类。在所有生态位等效性测试中，都拒绝了谱系 2 具有相同生态位的零假设，表明在遗传分化过程中存在生态位转移。相似性测试提供了生态位保守性的证据，表明谱系 2 共享更多的生态位空间特征，而不是随机预期的特征。生态位相似性和等效性测试表明，在 2 个谱系共享其生态空间的主要部分的复杂生态位模式中。谱系 B 的气候生态位代表了谱系 A 整个气候生态位空间的一个边缘和专门部分，具有更温暖的条件。造山作用引起的隔离和随后的生态位分化，以及局部适应，可能导致 L. reevesii 的遗传分化和进一步的谱系分类。
