Pak J Biol Sci. 2021 Jan;24(2):235-240. doi: 10.3923/pjbs.2021.235.240.
The increase of antibiotic-resistant bacteria is a problem for global health that needs to find new antibiotic drugs. The plant is the potential source of antibiotic substances that important to solve the antibiotic-resistant bacteria. This study was aimed to evaluate the antibacterial activity of Zamioculcas zamiifolia stem extracts against nine human pathogenic bacteria.
Z. zamiifolia stems were extracted with five extraction solvents. The screening of antibacterial activity of stem extract was measured using agar disc diffusion assay. The Minimal Inhibition Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values of extracts were determined using the broth microdilution assay and colorimetric assay.
The results indicated that the lowest MIC value of 0.09 mg mL-1 against Staphylococcus aureus TISTR 1466 was obtained from hexane extraction. The lowest MBCs value of 1.56 mg mL-1 against Bacillus cereus TISTR 2373, Listeria spp. and Escherichia coli TISTR 527 were obtained from ethanol and methanol extractions.
The ethanolic and methanolic stem extracts of Z. zamiifolia demonstrated the highest anti-human pathogenic bacterial activity. This is the first report to demonstrate the high potential of antibacterial substance from Z. zamiifolia stem extracts, which can be developed further as a natural drug for treating bacterial infectious diseases.
抗生素耐药菌的增加是一个全球性的健康问题，需要寻找新的抗生素药物。植物是抗生素物质的潜在来源，对于解决抗生素耐药菌问题非常重要。本研究旨在评估朱蕉茎提取物对 9 种人类致病菌的抗菌活性。
用 5 种提取溶剂提取朱蕉茎。采用琼脂扩散法筛选茎提取物的抗菌活性。采用肉汤微量稀释法和比色法测定提取物的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值。
结果表明，从正己烷提取物中获得了对金黄色葡萄球菌 TISTR 1466 的最低 MIC 值 0.09mg/mL。从乙醇和甲醇提取物中获得了对蜡样芽孢杆菌 TISTR 2373、李斯特菌和大肠杆菌 TISTR 527 的最低 MBC 值 1.56mg/mL。
朱蕉茎的乙醇和甲醇提取物表现出最高的抗人类致病菌活性。这是首次报道朱蕉茎提取物具有很强的抗菌物质潜力，可以进一步开发为治疗细菌感染性疾病的天然药物。
