Pak J Biol Sci. 2020 Jan;23(12):1676-1680. doi: 10.3923/pjbs.2020.1676.1680.
Commiphora gileadensis is a plant in the Burseraceae family that grows in the western area of Saudi Arabia. Traditionally, it is used in the treatment of some superficial infections.
The methanolic extract of Commiphora gileadensis isolated from its leaves and branches. The in vitro study was conducted to determine the effect of this extract on Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa using an agar diffusion and Minimum inhibitory concentration (MIC) methods. The in vivo study was conducted through two different methods. The first method, 20 male Balb c-1 mice were used for the determination of Commiphora gileadensis methanolic extract toxicity (LD50). In the second method, 40 male mice were used and were put into four groups. The first and second groups were injected subcutaneously with 108 CFU of MRSA 1 mL-1, while the third and fourth groups were injected with 108 CFU of Pseudomonas aeruginosa 1 mL-1. The comparison between groups was done by using a t-test (p<0.05).
The methanolic extract of Commiphora gileadensis had a greater sensitivity zone on MRSA and Pseudomonas aeruginosa, 7 and 3 mm respectively. The MIC of the extract was 1/8 and 1/2 for MRSA and Pseudomonas aeruginosa respectively. The in vivo study showed that the extract was non-toxic, it also showed that the extract decreased the mortality of mice induced by MRSA injection significantly (p<0.05) While insignificantly with Pseudomonas aeruginosa.
The total Commiphora gileadensis methanolic extract had an antibacterial effect on MRSA and Pseudomonas aeruginosa. This extract was non-toxic for the mice.
角茴香是一种生长在沙特阿拉伯西部的柏科植物，传统上用于治疗一些皮肤浅部感染。
从角茴香的叶和枝中分离出角茴香的甲醇提取物。体外研究采用琼脂扩散和最小抑菌浓度（MIC）法，观察该提取物对角茴香对耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌的影响。体内研究采用两种不同的方法。第一种方法，20 只雄性 Balb c-1 小鼠用于测定角茴香甲醇提取物的毒性（LD50）。第二种方法，将 40 只雄性小鼠分为 4 组。第一组和第二组皮下注射 108 CFU 1 mL-1 MRSA，第三组和第四组皮下注射 108 CFU 1 mL-1 铜绿假单胞菌。通过 t 检验（p<0.05）比较组间差异。
角茴香甲醇提取物对角茴香对 MRSA 和铜绿假单胞菌的敏感性更强，分别为 7 毫米和 3 毫米。提取物的 MIC 对 MRSA 和铜绿假单胞菌分别为 1/8 和 1/2。体内研究表明，该提取物无毒性，还表明该提取物显著降低了 MRSA 诱导的小鼠死亡率（p<0.05），而对铜绿假单胞菌则无显著影响。
角茴香总甲醇提取物对角茴香对 MRSA 和铜绿假单胞菌具有抗菌作用。该提取物对小鼠无毒性。
