Institute of Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, School of Medical Technology, Guangdong Medical University, Dongguan, 523808, China.
Biotherapy Center, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
J Hematol Oncol. 2020 Dec 4;13(1):166. doi: 10.1186/s13045-020-01005-x.
Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing to single-molecular, single-cell and spatial transcriptome approaches has enabled increasingly accurate, individual cell resolution incorporated with spatial information. Cancer, a major malignant and heterogeneous lethal disease, remains an enormous challenge in medical research and clinical treatment. As a vital tool, RNA sequencing has been utilized in many aspects of cancer research and therapy, including biomarker discovery and characterization of cancer heterogeneity and evolution, drug resistance, cancer immune microenvironment and immunotherapy, cancer neoantigens and so on. In this review, the latest studies on RNA sequencing technology and their applications in cancer are summarized, and future challenges and opportunities for RNA sequencing technology in cancer applications are discussed.
在过去的几十年中，RNA 测序技术取得了显著进展，成为了转录组分析的主要方法。从批量 RNA 测序到单分子、单细胞和空间转录组方法的变革，使得越来越精确的、具有个体细胞分辨率的技术与空间信息相结合成为可能。癌症是一种主要的恶性和异质性致死性疾病，仍然是医学研究和临床治疗中的巨大挑战。作为一种重要的工具，RNA 测序已被应用于癌症研究和治疗的多个方面，包括生物标志物的发现以及癌症异质性和进化、耐药性、癌症免疫微环境和免疫治疗、癌症新抗原等方面的特征描述。在这篇综述中，总结了 RNA 测序技术的最新研究进展及其在癌症中的应用，并讨论了 RNA 测序技术在癌症应用中的未来挑战和机遇。
