Chiba M, Ohta H, Iizuka M, Masamune O
First Department of Internal Medicine, Akita University School of Medicine, Japan.
Gastroenterol Jpn. 1987 Dec;22(6):703-8. doi: 10.1007/BF02776742.
Immunoglobulin (Ig)-containing cells were studied in the lamina propria of the normal human large bowel and around the lymph follicle, including Peyer's patches, in the normal intestine. Ig-containing cells were identified by the indirect immunoperoxidase staining method, using mouse anti-human Ig monoclonal antibodies. In the lamina propria in the large bowel, the mean percentage of IgA+ (IgA1+ cells and IgA2+ cells), IgM+, IgD+, IgG+ and IgE+ cells was 75.9, 8.5, 7.3, 5.8, 2.5, respectively (total 100), namely there was a marked preponderance of IgA+ cells in comparison to IgG+ cells. However, IgG+ cells were observed not only on the epithelial but also in the serosal side of lymph follicles, showing a ring-like pattern. Ig+ cells of the other four classes did not show such a pattern. The ring-like distribution of IgG+ cells around lymph follicles was observed in both the large and small intestine including Peyer's patches. This tendency was observed in 9 out of 14 follicles (64.3 per cent). A large number of IgG+ cells were observed outside lymph follicles, while a small number of IgG+ cells were observed at the most outer rim of lymph follicles which suggested a local maturation of IgG+ cells. The significance and the role of the newly recognized IgG+ cells in the vicinity of lymph follicles remain to be resolved.
在正常人大肠固有层以及正常肠道包括派尔集合淋巴结在内的淋巴滤泡周围，对含免疫球蛋白（Ig）的细胞进行了研究。使用小鼠抗人Ig单克隆抗体，通过间接免疫过氧化物酶染色法鉴定含Ig的细胞。在大肠固有层中，IgA +（IgA1 +细胞和IgA2 +细胞）、IgM +、IgD +、IgG +和IgE +细胞的平均百分比分别为75.9、8.5、7.3、5.8、2.5（总计100），即与IgG +细胞相比，IgA +细胞明显占优势。然而，不仅在淋巴滤泡的上皮侧，而且在浆膜侧都观察到了IgG +细胞，呈现出环状模式。其他四类Ig +细胞未呈现这种模式。在包括派尔集合淋巴结在内的大肠和小肠中均观察到淋巴滤泡周围IgG +细胞的环状分布。在14个滤泡中有9个（64.3%）观察到这种趋势。在淋巴滤泡外观察到大量IgG +细胞，而在淋巴滤泡最外缘观察到少量IgG +细胞，这表明IgG +细胞存在局部成熟现象。淋巴滤泡附近新发现的IgG +细胞的意义和作用仍有待阐明。
