Iizuka M
First Department of Internal Medicine, Akita University School of Medicine, Japan.
Gastroenterol Jpn. 1990 Feb;25(1):24-31. doi: 10.1007/BF02785326.
IgG subclass-containing cells in colonic mucosa were examined in three groups; 1) normal controls 2) cases of ulcerative colitis (UC) 3) cases of colitis excluding UC and Crohn's disease (non-IBD colitis) by indirect immunoperoxidase staining method using mouse anti-IgG subclass monoclonal antibodies. The numbers (and proportions) of IgG1, IgG2, IgG3, and IgG4-containing cells in normal colonic mucosa was 80 +/- 29/mm2 (44.6%), 44 +/- 21 (24.1%), 44 +/- 24 (23.7%), 13 +/- 10 (7.7%), respectively. The proportion of IgG subclass-containing cells in normal colonic mucosa was different from the known proportion of IgG subclass in serum. In UC, the numbers of all IgG subclasses-containing cells were significantly increased compared to controls and non-IBD colitis. However, only IgG1-containing cells were increased in proportion (50.3%) compared to normal controls. In non-IBD colitis, the numbers of IgG1- and IgG2-containing cells were increased compared to the controls, but the increases were less than UC, and there was no difference in the proportion of IgG subclass compared to normal controls. The differences in the numbers and in the proportions of IgG subclass-containing cells between UC and non-IBD colitis may reflect differences in the underlying disease process.
采用小鼠抗IgG亚类单克隆抗体的间接免疫过氧化物酶染色法，对三组对象的结肠黏膜中含IgG亚类的细胞进行了检测：1）正常对照组；2）溃疡性结肠炎（UC）病例；3）排除UC和克罗恩病的结肠炎病例（非IBD结肠炎）。正常结肠黏膜中含IgG1、IgG2、IgG3和IgG4的细胞数量（及比例）分别为80±29/mm²（44.6%）、44±21（24.1%）、44±24（23.7%）、13±10（7.7%）。正常结肠黏膜中含IgG亚类细胞的比例与血清中已知的IgG亚类比例不同。在UC中，与对照组和非IBD结肠炎相比，所有含IgG亚类细胞的数量均显著增加。然而，与正常对照组相比，仅含IgG1的细胞比例增加（50.3%）。在非IBD结肠炎中，与对照组相比，含IgG1和IgG2的细胞数量增加，但增加幅度小于UC，且与正常对照组相比，IgG亚类比例无差异。UC和非IBD结肠炎之间含IgG亚类细胞数量及比例的差异可能反映了潜在疾病过程的差异。
