组织形态计量学和免疫组织化学研究表明，硅酸三钙水泥与氧化锆或氧化铌联合应用是一种有前途的材料，可用于修复大鼠穿孔牙髓室底牙周组织。

Histomorphometric and immunohistochemical study shows that tricalcium silicate cement associated with zirconium oxide or niobium oxide is a promising material in the periodontal tissue repair of rat molars with perforated pulp chamber floors.

机构信息

Department of Restorative Dentistry, Dental School, São Paulo State University (UNESP), Araraquara, Brazil.

Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Laboratory of Histology and Embryology, Dental School, São Paulo State University (UNESP), Araraquara, Brazil.

出版信息

Int Endod J. 2021 May;54(5):736-752. doi: 10.1111/iej.13459. Epub 2021 Jan 9.

DOI:10.1111/iej.13459

PMID:33277697

Abstract

AIM

To evaluate the periodontium response to tricalcium silicate (TCS) with zirconium oxide (ZrO ) or niobium oxide (Nb O ) used in the sealing of perforated pulp chamber floors in rat maxillary molars.

METHODOLOGY

In eighty rats, the perforations in right maxillary molars were filled with either TCS + ZrO , TCS + Nb O , White MTA (used as a gold standard material) or no repair material was placed (Sham Group, SG); the left molars of SG, were used as controls (CG). Sections of maxillary fragments following 7, 15, 30 and 60 days were used to evaluate the volume densities of inflammatory cells (VvIC) and fibroblasts (VvFb), width of the periodontal space, amount of collagen, number of osteoclasts and number of IL-6-immunostained cells. The data were subjected to two-way ANOVA followed by Tukey's test (P ≤ 0.05).

RESULTS

At all periods, significant differences in VvIC were not detected among TCS + ZrO TCS + Nb O and MTA groups, which had values significantly lower (P < 0.05) than the SG. Significant differences in the number of IL-6-immunolabelled cells were not observed among TCS + ZrO , TCS + Nb O and MTA groups (P > 0.05) at 15, 30 and 60 days. At 7, 15 and 30 days, the number of osteoclast was significantly greater in TCS + ZrO TCS + Nb O and MTA (P < 0.05) than in the CG; no significant difference was detected after 60 days (P > 0.05). The width of the periodontal space and amount of collagen in TCS + ZrO and TCS + Nb O groups were similar to the CG at 30 and 60 days while SG specimens had a significant reduction (P < 0.05) in the amount of collagen and significant increase (P < 0.05) in the width of the periodontal space.

CONCLUSIONS

TCS + ZrO and TCS + Nb O were associated with periodontium repair since these materials allowed the reestablishment of periodontal space width and collagen formation when used in the filling of uninfected perforations in the pulp chamber floor of maxillary rat molars. Furthermore, the significant reduction in the periodontal space of TCS + ZrO and TCS + Nb O specimens after 60 days confirmed that the experimental materials were associated with a more rapid recovery of the injured tissues than MTA.

摘要

目的

评价三硅酸钙（TCS）分别与氧化锆（ZrO）或氧化铌（NbO）联合应用于大鼠上颌磨牙髓室底穿孔封闭时对牙周组织的反应。

方法

在 80 只大鼠中，右侧上颌磨牙的穿孔分别用 TCS+ZrO、TCS+NbO、White MTA（用作金标准材料）或不放置任何修复材料（Sham 组，SG）进行填充；SG 的左侧磨牙用作对照（CG）。在 7、15、30 和 60 天后，对上颌骨碎片的切片进行评估，以评估炎症细胞（VvIC）和成纤维细胞（VvFb）的体积密度、牙周间隙宽度、胶原含量、破骨细胞数量和 IL-6 免疫染色细胞数量。采用双因素方差分析（ANOVA），随后进行 Tukey 检验（P≤0.05）。

结果

在所有时间段，TCS+ZrO、TCS+NbO 和 MTA 组之间 VvIC 无显著差异，且明显低于 SG（P<0.05）。在 15、30 和 60 天时，TCS+ZrO、TCS+NbO 和 MTA 组之间 IL-6 免疫标记细胞的数量无显著差异（P>0.05）。在 7、15 和 30 天时，TCS+ZrO、TCS+NbO 和 MTA 组的破骨细胞数量明显多于 CG（P<0.05）；但在 60 天后无显著差异（P>0.05）。TCS+ZrO 和 TCS+NbO 组在 30 和 60 天时牙周间隙宽度和胶原含量与 CG 相似，而 SG 标本的胶原含量明显减少（P<0.05），牙周间隙宽度明显增加（P<0.05）。