LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal; Glycosciences Laboratory, Department of Metabolism, Digestion and Reproduction, Imperial College London, W12 0NN, UK.
UCIBIO, Department of Chemistry, School of Science and Technology, NOVA University of Lisbon, 2829-516 Lisbon, Portugal.
Carbohydr Polym. 2021 Feb 1;253:117350. doi: 10.1016/j.carbpol.2020.117350. Epub 2020 Nov 2.
The structural diversity of the lipopolysaccharides (LPSs) from Helicobacter pylori poses a challenge to establish accurate and strain-specific structure-function relationships in interactions with the host. Here, LPS structural domains from five clinical isolates were obtained and compared with the reference strain 26695. This was achieved combining information from structural analysis (GC-MS and ESI-MS) with binding data after interrogation of a LPS-derived carbohydrate microarray with sequence-specific proteins. All LPSs expressed Lewis and N-acetyllactosamine determinants. Ribans were also detected in LPSs from all clinical isolates, allowing their distinction from the 26695 LPS. There was evidence for 1,3-d-galactans and blood group H-type 2 sequences in two of the clinical isolates, the latter not yet described for H. pylori LPS. Furthermore, carbohydrate microarray analyses showed a strain-associated LPS recognition by the immune lectins DC-SIGN and galectin-3 and revealed distinctive LPS binding patterns by IgG antibodies in the serum from H. pylori-infected patients.
幽门螺杆菌的脂多糖（LPS）结构多样性对建立与宿主相互作用的准确和菌株特异性结构-功能关系提出了挑战。在这里，从五个临床分离株中获得了 LPS 结构域，并与参考菌株 26695 进行了比较。这是通过将结构分析（GC-MS 和 ESI-MS）的信息与结合数据相结合来实现的，结合数据是通过用序列特异性蛋白对 LPS 衍生的碳水化合物微阵列进行询问获得的。所有 LPS 都表达了 Lewis 和 N-乙酰乳糖胺决定簇。在所有临床分离株的 LPS 中也检测到了核糖，这使其与 26695 LPS 区分开来。有证据表明，在两个临床分离株中存在 1,3-d-半乳糖和血型 H 型 2 序列，后者尚未在幽门螺杆菌 LPS 中描述。此外，碳水化合物微阵列分析显示免疫凝集素 DC-SIGN 和半乳糖凝集素-3 对菌株相关 LPS 的识别，并揭示了幽门螺杆菌感染患者血清中 IgG 抗体的独特 LPS 结合模式。
