Research Unit Plasma Technology (RUPT), Department of Applied Physics, Faculty of Engineering and Architecture, Ghent University, Sint-Pietersnieuwstraat 41, 9000 Ghent, Belgium.
Research Unit Plasma Technology (RUPT), Department of Applied Physics, Faculty of Engineering and Architecture, Ghent University, Sint-Pietersnieuwstraat 41, 9000 Ghent, Belgium.
Carbohydr Polym. 2021 Feb 1;253:117211. doi: 10.1016/j.carbpol.2020.117211. Epub 2020 Oct 17.
Given the complex calcified nature of the fibrous bone tissue, a combinatorial approach merging specific topographical/biochemical cues was adopted to design bone tissue-engineered scaffolds. Coral having a Ca-enriched structure was added to electrospun chitosan (CS)/polyethylene oxide (PEO) nanofibers that were subjected to plasma surface modifications using a medium pressure Ar, air or N dielectric barrier discharge. Plasma incorporated oxygen- and nitrogen-containing functionalities onto the nanofibers surface thus enhancing their wettability. Plasma treatment enhanced the performance of osteoblasts and the interplay between plasma treatment and coral was shown to boost initial cell adhesion. The fibers capacity to trigger calcium phosphate growth was predicted via immersion in simulated body fluid. Globular carbonate apatite nanocrystals were deposited on plasma-treated CS/PEO NFs while thicker layers of flake-like nanocrystals were covering plasma-treated Coral/CS/PEO fibers without blocking the interfibrous pores. Overall, the exclusive multifaceted plasma-treated Coral/CS/PEO nanofibers are believed to revolutionize the bone tissue engineering field.
鉴于纤维状骨组织的复杂钙化特性，采用了一种组合方法，将特定的地形/生化线索结合起来，设计用于组织工程的骨支架。将富含 Ca 的珊瑚添加到静电纺丝的壳聚糖（CS）/聚氧化乙烯（PEO）纳米纤维中，然后使用中压 Ar、空气或 N 电介质阻挡放电对其进行等离子体表面改性。等离子体将含氧和含氮的官能团掺入纳米纤维表面，从而提高其润湿性。等离子体处理增强了成骨细胞的性能，并且等离子体处理和珊瑚之间的相互作用被证明可以促进初始细胞黏附。通过在模拟体液中浸泡来预测纤维触发磷酸钙生长的能力。球形碳酸磷灰石纳米晶体沉积在等离子体处理的 CS/PEO NF 上，而较厚的片状纳米晶体层覆盖等离子体处理的珊瑚/CS/PEO 纤维，而不会堵塞纤维间的孔。总的来说，独特的多方面等离子体处理的珊瑚/CS/PEO 纳米纤维有望彻底改变骨组织工程领域。
