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一种用于肺炎克雷伯菌和大肠杆菌质粒转化的快速高效方法。

A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli.

作者信息

Merrick M J, Gibbins J R, Postgate J R

机构信息

AFRC Unit of Nitrogen Fixation, University of Sussex, Brighton, UK.

出版信息

J Gen Microbiol. 1987 Aug;133(8):2053-7. doi: 10.1099/00221287-133-8-2053.

DOI:10.1099/00221287-133-8-2053
PMID:3327913
Abstract

A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae M5a1 and Escherichia coli K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl2 to facilitate DNA uptake, is substantially more efficient for K. pneumoniae M5a1 than the conventional transformation procedure for E. coli. The simplicity and speed of the method makes it very attractive for routine transformation of K. pneumoniae M5a1 and E. coli K12.

摘要

已开发出一种用于肺炎克雷伯菌M5a1和大肠杆菌K12质粒转化的快速高效方法。该方法在氯化钙存在下利用冻融循环促进DNA摄取，对肺炎克雷伯菌M5a1的效率比对大肠杆菌的传统转化程序高得多。该方法的简单性和速度使其对肺炎克雷伯菌M5a1和大肠杆菌K12的常规转化非常有吸引力。

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