Kim Kihyeun, Kim Hyeonghun, Jo Eun-Jung, Jang Hyungjun, Park Jiyoon, Jung Gun Young, Kim Min-Gon
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
Biosens Bioelectron. 2021 Mar 1;175:112855. doi: 10.1016/j.bios.2020.112855. Epub 2020 Nov 26.
The portability of electronic-based biosensors is limited because of the use of batteries and/or solutions containing reactants such as enzymes for assay, which limits the utility of such biosensors in point-of-care (POC) testing. In this study, we report on the development of a self-powered biosensor composed of only portable components: a reactant-containing poly (ethylene glycol) (PEG) film for the colorimetric assay, and a self-powered n-InGaZnO/p-Si photodetector. The PEG film containing enzymes and color-developing agents was formed on a glass slide by spin coating. The self-powered biosensor was fabricated by placing the hybrid film on the p-n junction photodetector, and applied in non-invasive glucose detection (salivary glucose). Injection of the target-containing solution dissolved the PEG that led to the release of enzymes and color-developing agents, resulting in a colorimetric assay. The colorimetric assay could attenuate the light reaching the photodetector, thus facilitating target concentration verification by measuring the photocurrent. Our self-powered biosensor has two main advantages: (i) all components of the biosensor are portable and (ii) dilution of target concentration is avoided as the reagents are in the PEG film. Therefore, the self-powered biosensor, without solution-phase components, could be highly beneficial for creating portable, sensitive biosensors for POC testing.
基于电子的生物传感器的便携性受到限制，因为其使用电池和/或含有诸如用于检测的酶等反应物的溶液，这限制了此类生物传感器在即时检测（POC）中的效用。在本研究中，我们报告了一种仅由便携式组件组成的自供电生物传感器的开发：用于比色测定的含反应物的聚乙二醇（PEG）薄膜和自供电的n-InGaZnO/p-Si光电探测器。通过旋涂在载玻片上形成含有酶和显色剂的PEG薄膜。通过将混合薄膜放置在p-n结光电探测器上制备自供电生物传感器，并将其应用于无创葡萄糖检测（唾液葡萄糖）。注入含靶标溶液会溶解PEG，从而导致酶和显色剂释放，进而进行比色测定。比色测定会减弱到达光电探测器的光，从而通过测量光电流便于验证靶标浓度。我们的自供电生物传感器有两个主要优点：（i）生物传感器的所有组件都是便携式的；（ii）由于试剂在PEG薄膜中，避免了靶标浓度的稀释。因此，没有溶液相组件的自供电生物传感器对于创建用于POC检测的便携式、灵敏生物传感器可能非常有益。
