Xiao Yao, Wang Xin, Li Enyou, Liu Yiping, Jiang Dandan, Li Mei, Chen Chuang, Li Haiyang
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, People's Republic of China.
Anal Chim Acta. 2021 Jan 15;1142:118-126. doi: 10.1016/j.aca.2020.10.043. Epub 2020 Nov 4.
Rapid and quantitative determination of blood propofol concentration is important for anesthesiologists to accurately control intraoperative propofol dose, timely monitor physiological statuses of patients and greatly improve the safety of surgery. Herein, a dopant-assisted negative photoionization ion mobility spectrometer with the optimized ionization region structure and the three-way inlet design was developed, increasing the generation ratio of the reactant ions O, and improving the ionization efficiency of propofol molecules. Besides, the addition of methanol-anisole solution during injection promoted the neutral desorption of propofol in blood, further improving the detection sensitivity by an order of magnitude, eliminating any sample pretreatment and effectively reducing the single analysis time to less than 1 min compared to the previous article. The dual calibration quantitative method, i.e. the method of calibrating the O concentration and the sample concentration changes during the entire process of detecting propofol through the integral value of M·O and the maximum signal intensity of O, successfully achieved accurate quantification of blood propofol. And the linear calibration curve of propofol was obtained with the range of 0.1-15 ng μL and with the limit of detection of 0.03 ng μL, which was fulfilled to conduct propofol determination throughout the perioperative period. Finally, this method was applied to clinically measure the blood propofol concentration in patients newly regained consciousness with concentrations ranging from 0.2 ng μL to 3 ng μL, and it turned out that the older patient had the lower propofol concentration in blood.
快速、定量地测定血液中丙泊酚浓度对于麻醉医生准确控制术中丙泊酚剂量、及时监测患者生理状态以及大幅提高手术安全性至关重要。在此，开发了一种具有优化电离区结构和三通进样设计的掺杂剂辅助负光电离离子迁移谱仪，提高了反应物离子O的生成比例，提升了丙泊酚分子的电离效率。此外，进样过程中添加甲醇 - 苯甲醚溶液促进了血液中丙泊酚的中性解吸，进一步将检测灵敏度提高了一个数量级，无需任何样品预处理，与前文相比，单次分析时间有效缩短至1分钟以内。双校准定量方法，即通过M·O的积分值和O的最大信号强度在校准检测丙泊酚的整个过程中对O浓度和样品浓度变化进行校准的方法，成功实现了血液中丙泊酚的准确定量。得到了丙泊酚的线性校准曲线，范围为0.1 - 15 ng μL，检测限为0.03 ng μL，满足围手术期全程进行丙泊酚测定的要求。最后，该方法应用于临床测量新苏醒患者血液中的丙泊酚浓度，浓度范围为0.2 ng μL至3 ng μL，结果显示老年患者血液中的丙泊酚浓度较低。
