Liu Ying, Zhang Xiao-Hui, Mi Wei-Dong, Zhou Ying-Lin, Zhang Chang-Sheng, Zhang Xin-Xiang
Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, China.
Anal Bioanal Chem. 2021 Jan;413(1):279-287. doi: 10.1007/s00216-020-02999-6. Epub 2020 Oct 26.
Propofol is a widely used intravenous anesthetic agent in sedation and general anesthesia. To improve the safety and maintain the depth of anesthesia, it is important to develop a rapid, sensitive, and reliable method to monitor the concentration of propofol in blood during anesthesia continuously. Here, we present a novel strategy based on paper spray ionization-mass spectrometry (PSI-MS) to detect propofol. Samples (in 10 μL) were mixed with methanol as protein precipitation solvent and 2,6-dimethylphenol as internal standard. Protein micro-precipitation was achieved with methanol by vortexing and centrifuging for 5 s each, and propofol was extracted to the supernatant. PSI-MS was performed in negative ionization mode, and MS signal lasted for 1 min. The analysis of a single sample was completed within 2 min. The area ratios of propofol to internal standard were calculated for quantification. Limit of detection of 5.5 ng mL and limit of quantification of 18.2 ng mL were achieved for propofol in whole blood. Calibration curve was linear in the range of 0.02-10 μg mL. The developed method was used successfully in monitoring the propofol concentration in 3 patients' whole blood during anesthesia, showing its further application in controlling and feeding-back target concentration infusion. Graphical abstract.
丙泊酚是一种广泛应用于镇静和全身麻醉的静脉麻醉剂。为提高安全性并维持麻醉深度，开发一种快速、灵敏且可靠的方法以在麻醉期间持续监测血液中丙泊酚的浓度至关重要。在此，我们提出一种基于纸喷雾电离质谱法（PSI-MS）检测丙泊酚的新策略。将样品（10 μL）与作为蛋白质沉淀溶剂的甲醇以及作为内标的2,6-二甲基苯酚混合。通过每次涡旋和离心5秒，用甲醇实现蛋白质微沉淀，丙泊酚被萃取到上清液中。PSI-MS在负离子模式下进行，质谱信号持续1分钟。单个样品的分析在2分钟内完成。计算丙泊酚与内标的面积比进行定量。全血中丙泊酚的检测限为5.5 ng/mL，定量限为18.2 ng/mL。校准曲线在0.02 - 10 μg/mL范围内呈线性。所开发的方法成功用于监测3例患者麻醉期间全血中的丙泊酚浓度，显示出其在控制和反馈目标浓度输注方面的进一步应用。图形摘要。
