Meizarini Asti, Aryati Aryati, Rianti Devi, Riawan Wibi, Puteri Astari
Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2020 Oct;13(10):2221-2225. doi: 10.14202/vetworld.2020.2221-2225. Epub 2020 Oct 27.
Reepithelialization can be described as the resurfacing of a wound with new epithelium in the process of healing, with the overlapping step from keratinocyte migration and proliferation to the tissue contraction. Zinc oxide-turmeric extract dressing has been proven to have anti-inflammatory properties, but its effectivity in the reepithelialization process is still unknown. This study aimed to determine the effect of a wound dressing consisting of zinc oxide and turmeric extract on wound reepithelialization by assessing the expression of cytokeratin 14 (CK14), epidermal growth factor receptor (EGFR), and epithelial cadherin (E-cadherin).
A total of 40 Wistar rats were randomized into four control and four treatment groups (n=5 per group). On day 1, a square-shaped full-thickness skin excision measuring 6×6 mm in size was created in the dorsal thoracic area of the rats, and the wounds were either dressed with a combination of zinc oxide and turmeric extract in the treatment groups or left undressed in the control groups. Then, the rats were sequentially sacrificed on days 3, 5, 7, and 14 to obtain subepithelial excision samples, which were subsequently subjected to immunohistochemistry analysis for the expression of CK14, EGFR, and E-cadherin to ascertain wound reepithelization. The data were tabulated and analyzed using a one-way analysis of variance and least significant difference test.
The highest expression levels of CK14, EGFR, and E-cadherin were observed on days 7 and 14 in the treatment and control groups, respectively. While the expression levels of these markers on day 7 were found to be significantly higher in the treatment than the control groups, no significant difference in the expression levels on day 14 was detected between the control and treatment groups (p<0.05).
A wound dressing consisting of zinc oxide and turmeric extract can help accelerate reepithelization in the wound healing process.
再上皮化可描述为伤口在愈合过程中由新上皮重新覆盖，经历从角质形成细胞迁移、增殖到组织收缩的重叠步骤。氧化锌 - 姜黄提取物敷料已被证明具有抗炎特性，但其在再上皮化过程中的有效性仍未知。本研究旨在通过评估细胞角蛋白14（CK14）、表皮生长因子受体（EGFR）和上皮钙黏蛋白（E - cadherin）的表达，确定由氧化锌和姜黄提取物组成的伤口敷料对伤口再上皮化的影响。
总共40只Wistar大鼠被随机分为四个对照组和四个治疗组（每组n = 5）。在第1天，在大鼠胸背部区域制作一个边长为6×6 mm的方形全层皮肤切除伤口，治疗组用氧化锌和姜黄提取物的组合敷料包扎伤口，对照组不包扎。然后，在第3、5、7和14天依次处死大鼠以获取皮下切除样本，随后对样本进行免疫组织化学分析以检测CK14、EGFR和E - cadherin的表达，从而确定伤口再上皮化情况。数据进行列表整理，并采用单因素方差分析和最小显著差异检验进行分析。
治疗组和对照组中，CK14、EGFR和E - cadherin的最高表达水平分别在第7天和第14天观察到。虽然发现这些标志物在第7天的表达水平治疗组显著高于对照组，但在第14天对照组和治疗组之间未检测到表达水平的显著差异（p < 0.05）。
由氧化锌和姜黄提取物组成的伤口敷料有助于加速伤口愈合过程中的再上皮化。
