Suhariningsih S, Astuti Suryani Dyah, Husen Saikhu Akhmad, Winarni Dwi, Rahmawati Dian Astri, Mukti Akhmad Taufiq, Putra Alfian Pramudita, Miftahussurur Muhammad
Department of Physics, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Biophysics and Medical Physics Research Group, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Vet World. 2020 Oct;13(10):2286-2293. doi: 10.14202/vetworld.2020.2286-2293. Epub 2020 Oct 31.
At present, diabetes is treated with oral antidiabetic medicines, such as sulfonylureas and thiazolidine, as well as insulin injection. However, these methods have several shortcomings. Therefore, alternatives for treating diabetes mellitus (DM) are needed. This study aims to determine the combined effect of magnetic and electric fields on blood sugar levels and the diameter of Langerhans islets of diabetic mice.
Induction of DM in mice was carried out by administering lard for 2 weeks and continued with an intraperitoneal injection of streptozotocin, dissolved in a 4.5 pH citrate buffer, and administered in a dose of 30 mg/kg bodyweight for 5 days. Treatments were used in combination with magnetic and electric fields using on/off infrared light. Blood samples were pipetted through the tip of mice's tails to establish the blood sugar level for each individual mouse. Histology preparation of the pancreas organ was affected using the histology standard as well as hematoxylin and eosin staining methods. Langerhans islet diameter data were analyzed using analysis of variance followed by Duncan's multiple range test. Data analysis was performed at ssssssss=0.05.
The results showed that the combined treatment of permanent magnetic and unidirectional electric fields (PS) caused changes in blood sugar levels that were not significantly different from the normal control group. The PS treatment improved the diameter of the Langerhans islets but not to a significant degree compared to other treatments.
The use of PS treatment is effective for reducing the blood sugar levels of diabetic mice and improving the diameter of their Langerhans islets.
目前，糖尿病的治疗方法包括使用口服抗糖尿病药物，如磺脲类和噻唑烷二酮类，以及注射胰岛素。然而，这些方法存在一些缺点。因此，需要治疗糖尿病（DM）的替代方法。本研究旨在确定磁场和电场对糖尿病小鼠血糖水平和胰岛直径的联合作用。
通过给小鼠喂食猪油2周，然后腹腔注射溶解在pH值为4.5的柠檬酸盐缓冲液中的链脲佐菌素，剂量为30mg/kg体重，连续5天，诱导小鼠患糖尿病。使用开/关红外光将磁场和电场结合用于治疗。通过小鼠尾巴尖端吸取血样，以确定每只小鼠的血糖水平。使用组织学标准以及苏木精和伊红染色方法对胰腺器官进行组织学制备。使用方差分析和邓肯多重范围检验分析胰岛直径数据。数据分析在α=0.05水平进行。
结果表明，永久磁场和单向电场联合治疗（PS）导致血糖水平变化，与正常对照组无显著差异。与其他治疗相比，PS治疗改善了胰岛直径，但改善程度不显著。
使用PS治疗可有效降低糖尿病小鼠的血糖水平并改善其胰岛直径。
