Brown C, Toh B H, Pedersen J S, Clarke F M, Mackay I R, Gust I
Department of Pathology and Immunology, Monash University Medical School, Melbourne.
Pathology. 1987 Oct;19(4):347-50. doi: 10.3109/00313028709103881.
Sera from 82 patients with acute or chronic hepatitis and 40 chronic carriers of hepatitis B were examined by ELISA and immunoblotting for reactivity with the glycolytic enzyme aldolase. The results of the ELISA tests, expressed as a percentage of a positive control, were compared to those obtained with sera from 39 patients with rubella, 11 with cytomegalovirus infection and 74 healthy subjects. The ELISA reaction with sera, expressed as mean +/- standard deviation was, for 15 patients with hepatitis A, 58.3 +/- 20.5%; 15 with hepatitis B, 59.5 +/- 42.18; 23 with hepatitis non-A, non-B 51.1 +/- 34.4%; 11 with HBsAg positive chronic active hepatitis, 70.1 +/- 31.5%; and 17 with autoimmune chronic active hepatitis, 66.8 +/- 21.4%. All values were significantly (p less than 0.05-p - less than 0.001) higher than those obtained with sera from carriers of hepatitis B surface antigen, 25.6 +/- 27.2%; rubella, 21.1 +/- 20.0%; cytomegalovirus infection, 19.2 +/- 27.8%; or healthy subjects, 20.9 +/- 16.2%. In two randomly selected sera, reactivity with aldolase by ELISA was neutralized by absorption with the enzyme. Selected sera showing reactivity by ELISA reacted by immunoblotting with aldolase. The findings suggest that acute or chronic liver damage may provoke the production of autoantibodies to aldolase.
采用酶联免疫吸附测定法（ELISA）和免疫印迹法检测了82例急慢性肝炎患者及40例慢性乙肝携带者血清与糖酵解酶醛缩酶的反应性。将ELISA检测结果（以阳性对照的百分比表示）与39例风疹患者、11例巨细胞病毒感染患者及74例健康受试者的血清检测结果进行了比较。ELISA法检测血清反应性，以均值±标准差表示，结果如下：15例甲型肝炎患者为58.3±20.5%；15例乙型肝炎患者为59.5±42.18%；23例非甲非乙型肝炎患者为51.1±34.4%；11例乙肝表面抗原阳性慢性活动性肝炎患者为70.1±31.5%；17例自身免疫性慢性活动性肝炎患者为66.8±21.4%。所有这些值均显著高于（p＜0.05 - p＜0.001）乙肝表面抗原携带者血清（25.6±27.2%）、风疹患者血清（21.1±20.0%）、巨细胞病毒感染患者血清（19.2±27.8%）或健康受试者血清（20.9±16.2%）的检测值。在两份随机选取的血清中，ELISA法检测的与醛缩酶的反应性可被该酶吸附中和。ELISA法显示有反应性的部分血清，经免疫印迹法检测也与醛缩酶发生反应。这些发现提示，急慢性肝损伤可能激发抗醛缩酶自身抗体的产生。
