Zhou Jin-Wei, Jia Ai-Qun, Tan Xiao-Juan, Chen Hong, Sun Bing, Huang Tian-Zi, He Yu, Li Pei-Li, Liu En-Qi
School of Food and Biology Engineering, Xuzhou University of Technology, Xuzhou, China.
School of Life and Pharmaceutical Sciences, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, China.
Front Microbiol. 2020 Nov 12;11:584767. doi: 10.3389/fmicb.2020.584767. eCollection 2020.
The impact of 1-(4-amino-2-hydroxyphenyl)ethanone (AHPE) from the metabolites of endophytic fungus on quorum sensing (QS) of was evaluated for the first time in this study. Exposure to AHPE at concentrations ranging from 12.5 to 50 μg/mL, the β-galactosidase activity, acyl-homoserine lactone level, swimming motility, chemotaxis, and flagella formation were significantly inhibited. qRT-PCR quantification combined with the docking analysis demonstrated that AHPE affected the QS system of by repressing the transcriptional levels of and rather than signal mimicry. H NMR-based metabolic analysis indicated that the metabolism of was notably disturbed with AHPE treatment. AHPE treatment also resulted in the enhanced oxidative stress in . The enhanced oxidative stress lead to the disorder of energy supply, protein synthesis, and nucleotide metabolism, and ultimately attenuated the pathogenicity of . Our study indicated that AHPE can serve as a potential pesticide to defend against .
本研究首次评估了内生真菌代谢产物1-(4-氨基-2-羟基苯基)乙酮(AHPE)对[具体菌名未给出]群体感应(QS)的影响。暴露于浓度为12.5至50μg/mL的AHPE时，β-半乳糖苷酶活性、酰基高丝氨酸内酯水平、游动性、趋化性和鞭毛形成均受到显著抑制。qRT-PCR定量分析结合对接分析表明，AHPE通过抑制[相关基因未给出]和[相关基因未给出]的转录水平而非信号模拟来影响[具体菌名未给出]的QS系统。基于1H NMR的代谢分析表明，AHPE处理显著扰乱了[具体菌名未给出]的代谢。AHPE处理还导致[具体菌名未给出]氧化应激增强。氧化应激增强导致能量供应、蛋白质合成和核苷酸代谢紊乱，最终减弱了[具体菌名未给出]的致病性。我们的研究表明，AHPE可作为一种潜在的农药来抵御[具体菌名未给出]。
