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水稻条纹病毒外壳蛋白介导的病毒抗性与RNA沉默相关。 （你提供的原文最后似乎不完整，少了具体的植物或生物名称等信息）

Rice Stripe Virus Coat Protein-Mediated Virus Resistance Is Associated With RNA Silencing in .

作者信息

Sun Feng, Hu Peng, Wang Wei, Lan Ying, Du Linlin, Zhou Yijun, Zhou Tong

机构信息

Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

出版信息

Front Microbiol. 2020 Nov 13;11:591619. doi: 10.3389/fmicb.2020.591619. eCollection 2020.

DOI:10.3389/fmicb.2020.591619
PMID:33281789
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7691420/
Abstract

Rice stripe virus (RSV) causes rice stripe disease, which is one of the most serious rice diseases in eastern Asian countries. It has been shown that overexpression of RSV coat protein (CP) in rice plants enhances resistance against virus infection. However, the detailed mechanism underlying RSV CP-mediated virus resistance remains to be determined. In this study, we show that both translatable and non-translatable RSV CP transgenic plants exhibited immunity to virus infection. By using deep sequencing analysis, transgene-derived small interfering RNAs (t-siRNAs) from non-translatable CP transgenic plants and virus-derived small interfering RNAs (vsiRNAs) mapping in the CP region from RSV-infected wild-type plants showed similar sequence distribution patterns, except for a significant increase in the abundance of t-siRNA reads compared with that of CP-derived vsiRNAs. To further test the correlation of t-siRNAs with RSV immunity, we developed RSV CP transgenic plants in an siRNA-deficient mutant background, and these CP transgenic plants showed the same sensitivity to RSV infection as non-transgenic plants. Together, our data indicate that the expression of RSV CP protein from a transgene is not a prerequisite for virus resistance and RSV CP-mediated resistance is mostly associated with the RNA silencing mechanism in plants.

摘要

水稻条纹病毒（RSV）引发水稻条纹病，该病是东亚国家最严重的水稻病害之一。研究表明，水稻植株中RSV外壳蛋白（CP）的过表达增强了对病毒感染的抗性。然而，RSV CP介导的病毒抗性的详细机制仍有待确定。在本研究中，我们发现可翻译和不可翻译的RSV CP转基因植株均对病毒感染表现出免疫性。通过深度测序分析，来自不可翻译CP转基因植株的转基因衍生小干扰RNA（t-siRNAs）与RSV感染的野生型植株中CP区域的病毒衍生小干扰RNA（vsiRNAs）显示出相似的序列分布模式，只是t-siRNA读数的丰度与CP衍生的vsiRNAs相比显著增加。为了进一步测试t-siRNAs与RSV免疫性的相关性，我们在一个siRNA缺陷突变体背景下培育了RSV CP转基因植株，这些CP转基因植株对RSV感染的敏感性与非转基因植株相同。总之，我们的数据表明，转基因中RSV CP蛋白的表达不是病毒抗性的先决条件，并且RSV CP介导的抗性主要与植物中的RNA沉默机制相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/941c25d0bfa0/fmicb-11-591619-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/2f5336411c9c/fmicb-11-591619-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/aa8cb8075ed6/fmicb-11-591619-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/a042505a5a75/fmicb-11-591619-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/cbe001f1b344/fmicb-11-591619-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/1149975799ad/fmicb-11-591619-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/941c25d0bfa0/fmicb-11-591619-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/2f5336411c9c/fmicb-11-591619-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/aa8cb8075ed6/fmicb-11-591619-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/a042505a5a75/fmicb-11-591619-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/cbe001f1b344/fmicb-11-591619-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/1149975799ad/fmicb-11-591619-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e76/7691420/941c25d0bfa0/fmicb-11-591619-g006.jpg

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