Yang Zhen, Yang Xue, Dong Shujia, Ge Yao, Zhang Xuenan, Zhao Xinjie, Han Ning
Shandong Provincial Key Laboratory of Microbial Engineering, School of Biologic Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2020 Nov 12;11:564385. doi: 10.3389/fpls.2020.564385. eCollection 2020.
Grape ( L.) is a fruit tree with high salt tolerance and high nutritional value, medicinal value, and economic value. Suberin in roots is characterized by long-chain fatty acids and is thought to be related to the salt tolerance of grape. The key enzyme in the fatty acid elongation process is β-ketoacyl-CoA synthase (KCS). The function and the regulatory mechanism of in response to salt stress in grape are unclear. In this study, was isolated from L. A real-time quantitative polymerase chain reaction analysis showed that salt stress enhanced transcription levels in grapes. Overexpression of increased the tolerance to salt stress in during the germination and seedling stages. The improved salt tolerance was the result of the combined contributions of multiple mechanisms including the regulation of expression of ion transporters and channels, accumulation of osmotic regulating substances, and maintenance of membrane stability. The results of this study are valuable information on plant salt tolerance and provide a theoretical basis for the molecular mechanism of grape salt tolerance.
葡萄（Vitis L.）是一种具有高耐盐性且营养价值、药用价值和经济价值高的果树。根中的木栓质以长链脂肪酸为特征，被认为与葡萄的耐盐性有关。脂肪酸延长过程中的关键酶是β-酮酰辅酶A合酶（KCS）。其在葡萄中响应盐胁迫的功能和调控机制尚不清楚。在本研究中，从葡萄（Vitis L.）中分离得到了它。实时定量聚合酶链反应分析表明，盐胁迫增强了葡萄中它的转录水平。它的过表达提高了葡萄在萌发和幼苗阶段对盐胁迫的耐受性。耐盐性的提高是多种机制共同作用的结果，包括离子转运体和通道表达的调控、渗透调节物质的积累以及膜稳定性的维持。本研究结果为植物耐盐性提供了有价值的信息，并为葡萄耐盐性的分子机制提供了理论依据。
