Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.
Food Funct. 2019 Apr 17;10(4):1797-1807. doi: 10.1039/c8fo02175a.
Several studies have shown that fresh grape and its derivatives contain phenolic compounds exhibiting antioxidant and health promoting effects, particularly in relation to the cardiovascular system. In this study, two methods were developed to characterize sixteen varieties of table and wine grapes: (1) a LC-MS method to identify major and minor phenolic compounds; and (2) a HPLC-DAD method to quantify the most representative compounds. Sixty-seven molecules belonging to different classes of phenolic compounds were identified: anthocyanins, flavan-3-ols, flavonols, stilbenes and organic acids. In parallel, the free radical scavenging activity and anti-inflammatory activities of the 16 grape varieties were evaluated. The results showed a good correlation between the total phenolic content and the biological activity. Extracts from Exalta and Albarossa grape varieties were the most active in reducing IL-8 release by gastric epithelial cells (IC50 = 8.48 μg mL-1 and 6.68 μg mL-1, respectively), a biomarker of inflammatory processes. The observed biological activities were mainly associated with skin and seed extracts/portions. The interest in studying table grapes and their non-fermented derivatives as sources of healthy compounds has increased in the last few years and our findings suggest that table grapes and their fresh derivatives, in addition to wine, could be involved in the health promoting effects of the Mediterranean diet.
已有多项研究表明，新鲜葡萄及其制品中含有的酚类化合物具有抗氧化和促进健康的作用，特别是对心血管系统有益。在这项研究中，我们开发了两种方法来对 16 种鲜食葡萄和酿酒葡萄进行分析：（1）使用 LC-MS 方法鉴定主要和次要酚类化合物；（2）使用 HPLC-DAD 方法定量分析最具代表性的化合物。鉴定出了 67 种属于不同酚类化合物类别的分子：花色苷、黄烷醇、黄酮醇、白藜芦醇和有机酸。同时，还评估了 16 个葡萄品种的自由基清除活性和抗炎活性。结果表明，总酚含量与生物活性之间存在良好的相关性。Exalta 和 Albarossa 葡萄品种的提取物在降低胃上皮细胞中 IL-8 释放方面最有效（IC50 分别为 8.48μg·mL-1和 6.68μg·mL-1），IL-8 是炎症过程的生物标志物。观察到的生物活性主要与果皮和种子提取物/部分有关。近年来，人们对研究鲜食葡萄及其非发酵衍生物作为健康化合物来源的兴趣有所增加，我们的研究结果表明，除了葡萄酒之外，鲜食葡萄及其新鲜衍生物可能也与地中海饮食的健康促进作用有关。
