Gong Sanqiang, Jin Xuejie, Xiao Yilin, Li Zhiyong
Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Front Plant Sci. 2020 Nov 17;11:585202. doi: 10.3389/fpls.2020.585202. eCollection 2020.
Ocean acidification and warming affect the growth and predominance of algae. However, the effects of ocean acidification and warming on the growth and gene transcription of thermo-tolerant algae are poorly understood. Here we determined the effects of elevated temperature (H) and acidification (A) on a recently discovered coral-associated thermo-tolerant alga by culturing it under two temperature settings (26.0 and 32.0°C) crossed with two pH levels (8.16 and 7.81). The results showed that the growth of was positively affected by H, A, and the combined treatment (AH). However, no superimposition effect of H and A on the growth of was observed under AH. The analysis of chlorophyll fluorescence, pigment content, and subcellular morphology indicated that the chloroplast morphogenesis (enlargement) along with the increase of chlorophyll fluorescence and pigment content of might be a universal mechanism for promoting the growth of . Transcriptomic profiles revealed the effect of elevated temperature on the response of to acidification involved in the down-regulation of photosynthesis- and carbohydrate metabolism-related genes but not the up-regulation of genes related to antioxidant and ubiquitination processes. Overall, this study firstly reports the growth, morphology, and molecular response of the thermo-tolerant alga to future climate changes, suggesting the predominance of S. in its associated corals and/or coral reefs in the future.
海洋酸化和变暖会影响藻类的生长和优势地位。然而，人们对海洋酸化和变暖对耐热藻类生长和基因转录的影响了解甚少。在此，我们通过在两种温度设置（26.0和32.0°C）与两种pH水平（8.16和7.81）交叉组合的条件下培养一种最近发现的与珊瑚相关的耐热藻类，确定了高温（H）和酸化（A）对其的影响。结果表明，该藻类的生长受到高温、酸化以及联合处理（AH）的积极影响。然而，在AH处理下未观察到高温和酸化对该藻类生长的叠加效应。叶绿素荧光、色素含量和亚细胞形态分析表明，该藻类叶绿体形态发生（增大）以及叶绿素荧光和色素含量的增加可能是促进其生长的普遍机制。转录组图谱揭示了高温对该藻类对酸化反应的影响，涉及光合作用和碳水化合物代谢相关基因的下调，但不涉及抗氧化和泛素化过程相关基因的上调。总体而言，本研究首次报道了这种耐热藻类对未来气候变化的生长、形态和分子反应，表明该藻类在未来与其相关的珊瑚和/或珊瑚礁中具有优势地位。
