Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland/Ministry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, China.
Key Laboratory of Integrated Pest Management of Crops in Central China, Ministry of Agriculture/Hubei Key Laboratory of Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Int J Mol Sci. 2023 Sep 18;24(18):14248. doi: 10.3390/ijms241814248.
Unknown functional domain (DUF) proteins constitute a large number of functionally uncharacterized protein families in eukaryotes. DUF724s play crucial roles in plants. However, the insight understanding of wheat is currently lacking. To explore the possible function of in wheat growth and development and stress response, the family members were systematically identified and characterized. In total, 14 were detected from a wheat reference genome; they are unevenly distributed across the 11 chromosomes, and, according to chromosome location, they were named to . Evolution analysis revealed that were under negative selection, and fragment replication was the main reason for family expansion. All TaDUF724s are unstable proteins; most TaDUF724s are acidic and hydrophilic. They were predicted to be located in the nucleus and chloroplast. The promoter regions of were enriched with the -elements functionally associated with growth and development, as well as being hormone-responsive. Expression profiling showed that was highly expressed in seedings, roots, leaves, stems, spikes and grains, and strongly expressed throughout the whole growth period. The 12 were post-transcription regulated by 12 wheat MicroRNA (miRNA) through cleavage and translation. RT-qPCR showed that six were regulated by biological and abiotic stresses. Conclusively, were systematically analyzed using bioinformatics methods, which laid a theoretical foundation for clarifying the function of in wheat.
未知功能域（DUF）蛋白构成了真核生物中大量功能尚未确定的蛋白质家族。DUF724 在植物中起着至关重要的作用。然而，目前对小麦的了解还很缺乏。为了探索 在小麦生长发育和应激响应中的可能功能，本研究系统地鉴定和分析了 家族成员。从一个小麦参考基因组中共检测到 14 个 ；它们在 11 条染色体上不均匀分布，根据染色体位置，分别命名为 到 。进化分析表明 受到负选择，片段复制是家族扩张的主要原因。所有 TaDUF724 都是不稳定的蛋白质；大多数 TaDUF724 是酸性和亲水的。它们被预测定位于细胞核和叶绿体。 的启动子区域富含与生长发育以及激素反应相关的功能的 - 元件。表达谱分析表明， 在幼苗、根、叶、茎、穗和籽粒中高度表达，在整个生长期间强烈表达。通过切割和翻译，12 个 被 12 个小麦 microRNA（miRNA）进行转录后调控。RT-qPCR 显示，六种 受到生物和非生物胁迫的调控。综上所述，使用生物信息学方法对 进行了系统分析，为阐明 在小麦中的功能奠定了理论基础。
