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养心定悸胶囊对大鼠心肌缺血的心脏保护作用机制

Mechanisms Underlying the Cardioprotection of YangXinDingJi Capsule against Myocardial Ischemia in Rats.

作者信息

Liu Miaomiao, Xue Yurun, Liang Yingran, Xue Yucong, Han Xue, Li Ziliang, Chu Li

机构信息

School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China.

Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation, Shijiazhuang 050091, China.

出版信息

Evid Based Complement Alternat Med. 2020 Nov 17;2020:8539148. doi: 10.1155/2020/8539148. eCollection 2020.

DOI:10.1155/2020/8539148
PMID:33281916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7685838/
Abstract

BACKGROUND

YangXinDingJi (YXDJ) capsule is one of traditional Chinese medicines (TCMs) derived from Zhigancao decoction, which is usually used for the treatment of cardiovascular disease in China. . Cardiovascular events are one of the leading causes of death worldwide. Myocardial ischemia (MI) severely reduces myocyte longevity and function. The YangXinDingJi (YXDJ) capsule has been used in the treatment of clinical cardiac disease in China. Nevertheless, the underlying cellular mechanisms for the benefits to heart function resulting from the use of this capsule are still unclear. The aim of this study was to evaluate the protective effects of the YXDJ on isoprenaline-induced MI in rats and to clarify its underlying myocardial protective mechanisms based on L-type calcium channels and myocardial contractility.

MATERIALS AND METHODS

Rats were randomly divided into five groups with ten rats in each group: (1) control; (2) ISO-induced model; (3) high-dose YXDJ (2.8 g/kg/day intraperitoneally for five days), (4) low-dose YXDJ (1.4 g/kg/day for five days); and (5) verapamil ( = 10 in each group). Isoproterenol (ISO) was injected subcutaneously for two consecutive days to induce the rat model of MI. Heart and biochemical parameters were obtained. The patch-clamp technique was used to observe the regulatory effects of YXDJ on the L-type calcium current (I) in isolated cardiomyocytes. An IonOptix MyoCam detection system was used to observe the contractility of YXDJ on isolated cardiomyocytes.

RESULTS

YXDJ caused a significant improvement in pathological heart morphology and alleviated oxidative stress and inflammatory responses. Exposure to YXDJ caused a decrease in blockade of I in a concentration-dependent manner.

CONCLUSIONS

The results indicate that YXDJ significantly inhibited inflammatory cytokine expressions, oxidative stress, and L-type Ca channels, and decreased contractility in isolated rat cardiomyocytes. These findings may be relevant to the cardioprotective efficacy of YXDJ.

摘要

背景

养心定悸（YXDJ）胶囊是源自炙甘草汤的中药之一，在中国常用于治疗心血管疾病。心血管事件是全球主要死因之一。心肌缺血（MI）会严重降低心肌细胞寿命和功能。YXDJ胶囊已在中国用于临床心脏病治疗。然而，该胶囊对心脏功能有益的潜在细胞机制仍不清楚。本研究旨在评估YXDJ对异丙肾上腺素诱导的大鼠心肌梗死的保护作用，并基于L型钙通道和心肌收缩性阐明其潜在的心肌保护机制。

材料与方法

将大鼠随机分为五组，每组十只：（1）对照组；（2）异丙肾上腺素诱导模型组；（3）高剂量YXDJ组（腹腔注射2.8 g/kg/天，共五天）；（4）低剂量YXDJ组（1.4 g/kg/天，共五天）；（5）维拉帕米组（每组n = 10）。连续两天皮下注射异丙肾上腺素以诱导大鼠心肌梗死模型。获取心脏和生化参数。采用膜片钳技术观察YXDJ对分离心肌细胞中L型钙电流（I）的调节作用。使用IonOptix MyoCam检测系统观察YXDJ对分离心肌细胞收缩性的影响。

结果

YXDJ使心脏病理形态有显著改善，减轻氧化应激和炎症反应。暴露于YXDJ导致I的阻滞呈浓度依赖性降低。

结论

结果表明，YXDJ显著抑制炎性细胞因子表达、氧化应激和L型钙通道，并降低分离的大鼠心肌细胞的收缩性。这些发现可能与YXDJ的心脏保护功效相关。

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