Lim Dong Wook, Jeon Hyejin, Kim Minji, Yoon Minseok, Jung Jonghoon, Kwon Sangoh, Cho Suengmok, Um Min Young
Research Division of Food Functionality, Korea Food Research Institute, Wanju 55365, Korea.
Division of Food Biotechnology, University of Science & Technology, Daejeon 34113, Korea.
Nutr Res Pract. 2020 Dec;14(6):568-579. doi: 10.4162/nrp.2020.14.6.568. Epub 2020 Jul 2.
BACKGROUD/OBJECTIVES: Hepatic steatosis is the most common liver disorder, particularly in postmenopausal women. This study investigated the protective effects of standardized rice bran extract (RBS) on ovariectomized (OVX)-induced hepatic steatosis in rats.
MATERIALS/METHODS: HepG2 cells were incubated with 200 µM oleic acid to induce lipid accumulation with or without RBS and γ-oryzanol. OVX rats were separated into three groups and fed a normal diet (ND) or the ND containing 17β-estradiol (E2; 10 µg/kg) and RBS (500 mg/kg) for 16 weeks.
RBS supplementation improved serum triglyceride and free fatty acid levels in OVX rats. Histological analysis showed that RBS significantly attenuated hepatic fat accumulation and decreased hepatic lipid, total cholesterol, and triglyceride levels. Additionally, RBS suppressed the estrogen deficiency-induced upregulation of lipogenic genes, such as sterol regulatory element-binding protein 1 (), acetyl-CoA carboxylase 1, fatty acid synthase, glycerol-3-phosphate acyltransferase, and stearoyl-CoA desaturase 1.
RBS and γ-oryzanol effectively reduced lipid accumulation in a HepG2 cell hepatic steatosis model. RBS improves OVX-induced hepatic steatosis by regulating the -mediated activation of lipogenic genes, suggesting the benefits of RBS in preventing fatty liver in postmenopausal women.
背景/目的：肝脂肪变性是最常见的肝脏疾病，在绝经后女性中尤为常见。本研究调查了标准化米糠提取物（RBS）对去卵巢（OVX）诱导的大鼠肝脂肪变性的保护作用。
材料/方法：将HepG2细胞与200μM油酸一起孵育，以在有或没有RBS和γ-谷维素的情况下诱导脂质积累。将OVX大鼠分为三组，分别给予正常饮食（ND）或含17β-雌二醇（E2；10μg/kg）和RBS（500mg/kg）的ND，持续16周。
补充RBS可改善OVX大鼠的血清甘油三酯和游离脂肪酸水平。组织学分析表明，RBS可显著减轻肝脏脂肪堆积，并降低肝脏脂质、总胆固醇和甘油三酯水平。此外，RBS抑制了雌激素缺乏诱导的脂质生成基因的上调，如固醇调节元件结合蛋白1、乙酰辅酶A羧化酶1、脂肪酸合酶、甘油-3-磷酸酰基转移酶和硬脂酰辅酶A去饱和酶1。
RBS和γ-谷维素在HepG2细胞肝脂肪变性模型中有效降低了脂质积累。RBS通过调节脂质生成基因的介导激活来改善OVX诱导的肝脂肪变性，表明RBS在预防绝经后女性脂肪肝方面具有益处。
