Katende J M, Nantulya V M, Musoke A J
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Trans R Soc Trop Med Hyg. 1987;81(4):607-8. doi: 10.1016/0035-9203(87)90425-1.
Trypanosoma brucei brucei MiTat 1.2 bloodstream and corresponding procyclic forms, as well as procyclics of T. b. gambiense and T. b. rhodesiense, were fixed, in suspension, using a mixture of 80% acetone and 0.25% (v/v) formalin in saline and used as antigens for diagnosis of African human trypanosomiasis by the indirect immunofluorescent antibody test. T. b. brucei bloodstream forms detected 41/42 and 37/41 parasitologically diagnosed cases of T. b. rhodesiense and T. b. gambiense trypanosomiasis, respectively, while the procyclic stages detected the same number (41/42) of T. b. rhodesiense, but fewer (29/41) T. b. gambiense infections.
布氏布氏锥虫MiTat 1.2血流型及相应的前循环型，以及布氏冈比亚锥虫和布氏罗德西亚锥虫的前循环型，在悬浮状态下，用80%丙酮和0.25%（v/v）福尔马林的生理盐水混合物固定，并用作抗原，通过间接免疫荧光抗体试验诊断非洲人类锥虫病。布氏布氏锥虫血流型分别检测出41/42例和37/41例经寄生虫学诊断的布氏罗德西亚锥虫病和布氏冈比亚锥虫病病例，而前循环期检测出相同数量（41/42）的布氏罗德西亚锥虫病病例，但布氏冈比亚锥虫感染病例较少（29/41）。
