评估体外转化技术以区分伊氏锥虫与循环传播的锥虫种群。
Evaluation of the in vitro transformation technique to distinguish Trypanosoma evansi from cyclically transmitted Trypanozoon stocks.
作者信息
Olaho-Mukani W, Mukunza F, Kimani J K, Njoka P K, Walubengo J
机构信息
Kenya Trypanosomiasis Research Institute, Kikuyu.
出版信息
Trop Med Parasitol. 1993 Jun;44(2):108-10.
In order to initiate transformation into procyclic forms, bloodstream trypanosomes, were transferred to semi-defined medium at 27 degrees C. All stocks previously classified as Trypanosoma brucei rhodesiense, T.b. gambiense and T.b. brucei transformed into procyclic forms. None of the 31 characterized T. evansi stocks transformed into procyclic forms, but died between day 4 and day 6 in culture. On the other hand, 3 out of 64 stocks of monomorphic brucei subgroup field isolates from camels transformed into procyclic forms, confirming the existence of T.b. brucei infection in camels kept close to tsetse belts.
为了启动向原循环型的转化，将血流型锥虫在27摄氏度转移至半限定培养基中。所有先前分类为布氏罗得西亚锥虫、布氏冈比亚锥虫和布氏布氏锥虫的菌株都转化为了原循环型。31株已鉴定的伊氏锥虫菌株均未转化为原循环型，而是在培养的第4天至第6天之间死亡。另一方面，来自骆驼的64株单形布氏锥虫亚组野外分离株中有3株转化为原循环型，证实了在靠近采采蝇带的骆驼中存在布氏布氏锥虫感染。
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