Nordlinger B, Wang S R, Bouma M E, Verthier N, Hillan K, Delelo R, Infante R
INSERM U.9, Hôpital Saint-Antoine, Paris, France.
Eur Surg Res. 1987;19(6):381-7. doi: 10.1159/000128726.
The aim of this work was to study hepatocyte multiplication after transplantation into the spleen, in order to apply this technique to the treatment of chronic liver disease. Hepatocytes isolated by an in situ collagenase perfusion technique in Wistar Furth rats were injected into the splenic parenchyma of three groups of syngeneic rats: controls with normal liver (group 1), 75% hepatectomies (group 2), and end-to-side portacaval shunts (group 3). The proliferation of transplanted hepatocytes was studied by autohistoradiography after the intraperitoneal administration of 0.6 microCi/g body weight of [3H]-thymidine, at 1, 3, 7 and 15 days after transplantation of hepatocytes. Significant incorporation of [3H]-thymidine by the transplanted hepatocytes during the study period was observed mostly in groups 2 and 3. The incorporation, although delayed was sustained and of greatest magnitude in the portacaval-shunted animals. The ability of transplanted hepatocytes to proliferate in the spleen, particularly after a portacaval shunt, indicates that this procedure may have therapeutic applications in the treatment of chronic liver disease.
这项工作的目的是研究肝细胞移植到脾脏后的增殖情况，以便将该技术应用于慢性肝病的治疗。通过原位胶原酶灌注技术从Wistar Furth大鼠分离出的肝细胞，被注入三组同基因大鼠的脾实质中：正常肝脏对照组（第1组）、75%肝切除组（第2组）和端侧门腔分流组（第3组）。在肝细胞移植后1、3、7和15天，腹腔注射每克体重0.6微居里的[3H] - 胸腺嘧啶核苷后，通过自动放射自显影术研究移植肝细胞的增殖情况。在研究期间，主要在第2组和第3组观察到移植肝细胞对[3H] - 胸腺嘧啶核苷的显著掺入。在门腔分流的动物中，虽然掺入延迟，但持续存在且程度最大。移植肝细胞在脾脏中增殖的能力，特别是在门腔分流后，表明该程序可能在慢性肝病的治疗中具有治疗应用价值。
