Esiri M M, Reading M C
Department of Neuropathology, Radcliffe Infirmary, Oxford, UK.
Neuropathol Appl Neurobiol. 1987 Nov-Dec;13(6):451-65. doi: 10.1111/j.1365-2990.1987.tb00074.x.
The macrophage population within and outside plaques from eight cases of multiple sclerosis (MS) (two clinically acute, four chronic progressive and two chronic non-progressive) has been examined in fresh frozen sections with a panel of monoclonal antibodies of macrophage, monocyte and MHC class II specificity. The majority of cells in active, hypercellular plaques, and at active borders, reacted with macrophage- and class II MHC-specific antibodies, and such cells extended beyond the border between demyelinated and myelinated parenchyma. In inactive plaques such cells that reacted with macrophage-specific antibodies were sparse and reacted only inconstantly with class II MHC-specific antibodies. Macrophage heterogeneity was evident in as much as one macrophage antibody, RFD7, reacted only with perivascular and not parenchymal macrophages in most plaques, but reacted with a variable proportion of parenchymal macrophages in active plaques. It is suggested that the RFD7 antibody may identify a sub-population of acute plaques, and that its use may clarify interpretation of findings related to other inflammatory cell populations by providing greater precision of classification of active plaques.
利用一组具有巨噬细胞、单核细胞和MHC II类特异性的单克隆抗体，对8例多发性硬化症（MS）（2例临床急性、4例慢性进展性和2例慢性非进展性）斑块内外的巨噬细胞群体进行了新鲜冰冻切片检查。在活跃的、细胞增多的斑块以及活跃边界处，大多数细胞与巨噬细胞和II类MHC特异性抗体发生反应，并且这些细胞延伸至脱髓鞘和有髓实质之间的边界之外。在不活跃的斑块中，与巨噬细胞特异性抗体发生反应的此类细胞稀少，并且仅偶尔与II类MHC特异性抗体发生反应。巨噬细胞的异质性很明显，因为一种巨噬细胞抗体RFD7在大多数斑块中仅与血管周围巨噬细胞而非实质巨噬细胞发生反应，但在活跃斑块中与不同比例的实质巨噬细胞发生反应。有人提出，RFD7抗体可能识别出急性斑块的一个亚群，并且使用它可能通过更精确地分类活跃斑块来阐明与其他炎症细胞群体相关的研究结果的解释。
