State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi, People's Republic of China.
J Appl Microbiol. 2021 Jul;131(1):470-484. doi: 10.1111/jam.14952. Epub 2020 Dec 18.
This study evaluated the effects of Bacillus amyloliquefaciens TL106, isolated from Tibetan pigs' faeces, on the growth performance, immune response, intestinal barrier function, morphology of jejunum, caecum and colon, and gut microbiota in the mice with enterohaemorrhagic Escherichia coli (EHEC)-induced intestinal diseases.
In all, 40 female C57BL/6J mice were randomly divided into four groups: mice fed a normal diet (Control), mice oral administration of TL106 daily (Ba), mice challenged with EHEC O157:H7 on day 15 (O157) and mice oral administration of TL106 daily and challenged with EHEC O157:H7 on day 15 (Ba+O157). The TL106 was administrated to mice for 14 days, and mice were infected with O157:H7 at day 15. We found that TL106 could prevent the weight loss caused by O157:H7 infection and alleviated the associated increase in pro-inflammatory factors (TNF-α, IL-1β, IL-6 and IL-8) and decrease in anti-inflammatory factor (IL-10) in serum and intestinal tissues of mice caused by O157:H7 infection (P < 0·05). Additionally, TL106 could prevent disruption of gut morphology caused by O157:H7 infection, and alleviate the associated decrease in expression of tight junction proteins (ZO-1, occludin and claudin-1) in jejunum and colon (P < 0·05). In caecum and colon, the alpha diversity for bacterial community analysis of Chao and ACE index in Ba+O157 group were higher than O157 group. The TL106 stabilized gut microbiota disturbed by O157:H7, including increasing Lachnospiraceae, Prevotellaceae, Muribaculaceae and Akkermansiaceae, and reducing Lactobacillaceae.
We indicated the B. amyloliquefaciens TL106 can effectively protect mice against EHEC O157:H7 infection by relieving inflammation, improving intestinal barrier function, mitigating permeability disruption and stabilizing the gut microbiota.
Bacillus amyloliquefaciens TL106 can prevent and treat intestinal disease induced by EHEC O157:H7 in mice, which may be a promising probiotic for disease prevention in animals.
本研究评估了从藏猪粪便中分离的解淀粉芽胞杆菌 TL106 对感染产志贺样毒素大肠杆菌（EHEC）O157：H7 诱导的肠道疾病的小鼠生长性能、免疫反应、肠道屏障功能、空肠、盲肠和结肠形态以及肠道微生物群的影响。
共 40 只雌性 C57BL/6J 小鼠随机分为四组：正常饮食喂养的小鼠（对照）、每日口服 TL106 的小鼠（Ba）、第 15 天感染 EHEC O157：H7 的小鼠（O157）和每日口服 TL106 并在第 15 天感染 EHEC O157：H7 的小鼠（Ba+O157）。TL106 处理小鼠 14 天，第 15 天感染 O157：H7。我们发现，TL106 可预防 O157：H7 感染引起的体重减轻，并减轻 O157：H7 感染引起的血清和肠道组织中促炎因子（TNF-α、IL-1β、IL-6 和 IL-8）的增加和抗炎因子（IL-10）的减少（P<0.05）。此外，TL106 可防止 O157：H7 感染引起的肠道形态破坏，并减轻 O157：H7 感染引起的空肠和结肠紧密连接蛋白（ZO-1、occludin 和 claudin-1）表达降低（P<0.05）。在盲肠和结肠中，Ba+O157 组的 Chao 和 ACE 指数的细菌群落分析的 alpha 多样性高于 O157 组。TL106 稳定了 O157：H7 扰乱的肠道微生物群，包括增加 Lachnospiraceae、Prevotellaceae、Muribaculaceae 和 Akkermansiaceae，并减少了 Lactobacillaceae。
我们表明，解淀粉芽胞杆菌 TL106 可通过缓解炎症、改善肠道屏障功能、减轻通透性破坏和稳定肠道微生物群，有效保护小鼠免受产志贺样毒素大肠杆菌 O157：H7 感染。
解淀粉芽胞杆菌 TL106 可预防和治疗小鼠感染产志贺样毒素大肠杆菌 O157：H7 引起的肠道疾病，这可能是一种有前途的动物疾病预防益生菌。
