Key Laboratory of Tropical Animal Breeding and Epidemic Disease Research of Hainan Province , Hainan University , Haikou , Hainan 570228 , People's Republic of China.
Key Laboratory of Tropical Biological Resources of Ministry of Education , Haikou , Hainan 570228 , People's Republic of China.
J Agric Food Chem. 2019 Apr 10;67(14):3932-3945. doi: 10.1021/acs.jafc.9b00861. Epub 2019 Mar 28.
Lactoferricin (Lfcin) B, derived from lactoferrin in whey, has attracted considerable attention because of its multiple biological functions. Zoonotic enterohemorrhagic Escherichia coli (EHEC) O157:H7 has adverse effects on intestinal epithelial barrier function, leading to serious intestinal disease. In this study, the EHEC O157:H7-induced intestinal dysfunction model was developed to investigate the effects of Lfcin B on EHEC O157:H7-induced epithelial barrier disruption and microbiota dysbiosis. Results showed that the inflammatory infiltration indexes in the jejunum of Lfcin B-treated animals were significantly decreased. Lfcin B administration also significantly improved ZO-1 and occludin expression following O157:H7-induced injury. Finally, microbiota analysis of the cecal samples revealed that Lfcin B inhibited the O157:H7-induced abnormal increase in Bacteroides. Therefore, Lfcin B efficiently attenuated O157:H7-induced epithelial barrier damage and dysregulation of inflammation status, while maintaining microbiota homeostasis in the intestine, indicating that it may be an excellent food source for prevention and therapy of EHEC O157:H7-related intestinal dysfunction.
乳铁素 B（Lfcin B）来源于乳清中的乳铁蛋白，因其多种生物学功能而受到广泛关注。人畜共患肠出血性大肠杆菌（EHEC）O157：H7 对肠道上皮屏障功能有不良影响，导致严重的肠道疾病。在本研究中，建立了 EHEC O157：H7 诱导的肠道功能障碍模型，以研究 Lfcin B 对 EHEC O157：H7 诱导的上皮屏障破坏和微生物失调的影响。结果表明，Lfcin B 处理动物的空肠炎症浸润指标显著降低。Lfcin B 给药还显著改善了 O157：H7 损伤后 ZO-1 和闭合蛋白的表达。最后，盲肠样本的微生物组分析显示，Lfcin B 抑制了 O157：H7 诱导的拟杆菌异常增加。因此，Lfcin B 有效减轻了 O157：H7 诱导的上皮屏障损伤和炎症状态失调，同时维持了肠道微生物组的平衡，表明它可能是预防和治疗 EHEC O157：H7 相关肠道功能障碍的一种极好的食物来源。
