College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
Vector Borne Zoonotic Dis. 2021 Feb;21(2):105-109. doi: 10.1089/vbz.2020.2658. Epub 2020 Dec 7.
, an intracellular zoonotic parasite, can infect humans and various animals worldwide. Wild rodents plan an important role as intermediate hosts of . Some studies on from wild rodents have been published, but the investigation data of in wild rodents in China are limited. Therefore, brain tissue samples from 382 wild rodents in four provinces and one autonomous region of China were screened by PCR amplification of B1 gene. Furthermore, the wild rodents were identified as five species based on their morphological characteristics, including ( = 35 from Heilongjiang), ( = 81 from Inner Mongolia), ( = 12 from Heilongjiang), ( = 2 from Heilongjiang; = 99 from Zhejiang; = 54 from Shanxi), and ( = 99 from Guangxi). The overall prevalence of in these wild rodents was 5.24% (20/382) in this study. At different regions, the highest prevalence of was in Guangxi (12.12%) compared with other regions (0% in Heilongjiang; 2.47% in Inner Mongolia; 2.02% in Zhejiang; 7.41% in Shanxi). At different climates, the higher prevalence was found in temperate/mesothermal climates (7.07%) compared with continental/microthermal climates (3.26%). Also, the results showed that had the highest prevalence of infection (12.12%) among the rodent species sampled. Moreover, sampling year was significantly related to the prevalence of in investigated wild rodents ( = 0.0117). This is the first report of in wild rodents from Zhejiang, Guangxi, Shanxi, Heilongjiang provinces and Inner Mongolia autonomous region in China, providing the fundamental information for further prevention and control of toxoplasmosis in China.
刚地弓形虫，一种细胞内的人畜共患寄生虫，可感染全球范围内的人类和各种动物。野生啮齿动物作为刚地弓形虫的中间宿主起着重要作用。已经有一些关于来自野生啮齿动物的刚地弓形虫的研究发表，但中国野生啮齿动物中刚地弓形虫的调查数据有限。因此，本研究通过 B1 基因的 PCR 扩增筛选了来自中国四个省和一个自治区的 382 只野生啮齿动物的脑组织样本。此外，根据形态学特征将这些野生啮齿动物鉴定为五个物种，包括 （ = 35 只来自黑龙江）、 （ = 81 只来自内蒙古）、 （ = 12 只来自黑龙江）、 （ = 2 只来自黑龙江； = 99 只来自浙江； = 54 只来自山西）和 （ = 99 只来自广西）。在这些野生啮齿动物中，刚地弓形虫的总感染率为 5.24%（20/382）。在不同地区，广西的感染率最高（12.12%），而其他地区的感染率为黑龙江（0%）、内蒙古（2.47%）、浙江（2.02%）和山西（7.41%）。在不同气候下，温带/中温气候的感染率较高（7.07%），而大陆/微温气候的感染率较低（3.26%）。此外，结果表明，在所采样的啮齿动物物种中， 具有最高的刚地弓形虫感染率（12.12%）。而且，采样年份与所调查的野生啮齿动物的刚地弓形虫感染率显著相关（ = 0.0117）。这是中国浙江、广西、山西、黑龙江省和内蒙古自治区首次报道野生啮齿动物感染刚地弓形虫，为中国进一步预防和控制弓形虫病提供了基础信息。
