Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036, Arcavacata di Rende (CS), Italy.
Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036, Arcavacata di Rende (CS), Italy.
Environ Pollut. 2021 Jan 15;269:116177. doi: 10.1016/j.envpol.2020.116177. Epub 2020 Nov 30.
Bisphenol A (BPA) is a contaminant whose presence in aquatic environments is increasing. In fish embryos and larvae, it severely affects cardiac development; however, its influence on the heart function of adult fish has been scarcely analyzed. This study investigated the effects of the in vivo exposure to BPA on heart physiology, morphology, and oxidative balance in the goldfish Carassius auratus. Adult fish were exposed for 4 and 10 days to two BPA concentrations (10 μM and 25 μM). Ex vivo working heart preparations showed that high concentrations of BPA negatively affected cardiac hemodynamics, as revealed by an impaired Frank-Starling response. This was paralleled by increased cardio-somatic indices and by myocardial structural changes. An altered oxidative status and a modulation of stress (HSPs) and pro-apoptotic (Bax and Cytochrome C) proteins expression were also observed in the heart of animals exposed to BPA, with detrimental effects at the highest concentration and the longest exposure time. Results suggest that, in the adult goldfish, BPA may induce stressful conditions to the heart with time- and concentration-dependent deleterious morpho-functional alterations.
双酚 A（BPA）是一种污染物，其在水生环境中的存在正在增加。在鱼类胚胎和幼体中，它会严重影响心脏发育；然而，其对成鱼心脏功能的影响却鲜有分析。本研究调查了体内暴露于 BPA 对金鱼（Carassius auratus）心脏生理学、形态和氧化平衡的影响。成年鱼分别暴露于两种 BPA 浓度（10 μM 和 25 μM）4 天和 10 天。离体工作心脏制剂显示，高浓度的 BPA 对心脏血液动力学产生负面影响，表现为 Frank-Starling 反应受损。这与心体指数增加和心肌结构变化平行。在暴露于 BPA 的动物心脏中还观察到氧化状态的改变以及应激（HSPs）和促凋亡（Bax 和 Cytochrome C）蛋白表达的调节，在最高浓度和最长暴露时间下具有有害影响。结果表明，在成年金鱼中，BPA 可能会随着时间和浓度的依赖性产生有害的形态和功能改变，从而对心脏造成压力。
