Naupu Paulina N, van Zyl Albertha R, Rybicki Edward P, Hitzeroth Inga I
Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa.
Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch 7701, South Africa.
Vaccines (Basel). 2020 Dec 6;8(4):740. doi: 10.3390/vaccines8040740.
Cervical cancer is ranked fourth among the top cancers in women and is the second most common cancer in low- and middle-income regions, with ~570,000 new cases reported in 2018, which attributed to 84% of worldwide cervical cancer cases. Three commercially available prophylactic Human papillomavirus (HPV) vaccines are effective at preventing HPV infections. However, these vaccines are expensive due to their complex production systems, therefore limiting their use in developing countries. Recently, the use of plants to produce vaccines has emerged as a cost-effective alternative to conventionally used expression systems. Here, L1 proteins of eight high-risk (HPV 16, 18, 31, 33, 35, 45, 52, and 58) and two low risk (HPV 6 and 34) HPV types were successfully expressed in and transmission electron microscopy (TEM) analysis showed the presence of VLPs and/or capsomeres. Immunogenicity studies were conducted in mice utilizing HPV 35, 52, and 58 and showed that type-specific L1-specific antibodies were produced which were able to successfully neutralize homologous HPV pseudovirions in pseudovirion-based neutralization assays (PBNAs). This work demonstrated the potential for using plant-based transient expression systems to produce affordable and immunogenic HPV vaccines, particularly for developing countries.
宫颈癌在女性最常见癌症中排名第四，是低收入和中等收入地区第二常见的癌症，2018年报告了约57万例新病例，占全球宫颈癌病例的84%。三种市售的预防性人乳头瘤病毒（HPV）疫苗对预防HPV感染有效。然而，由于其复杂的生产系统，这些疫苗价格昂贵，因此限制了它们在发展中国家的使用。最近，利用植物生产疫苗已成为传统使用的表达系统的一种经济有效的替代方案。在这里，8种高危（HPV 16、18、31、33、35、45、52和58）和2种低危（HPV 6和34）HPV类型的L1蛋白在[具体植物名称未给出]中成功表达，透射电子显微镜（TEM）分析显示存在病毒样颗粒（VLPs）和/或衣壳粒。利用HPV 35、52和58在小鼠中进行了免疫原性研究，结果表明产生了型特异性L1特异性抗体，这些抗体能够在基于假病毒的中和试验（PBNAs）中成功中和同源HPV假病毒。这项工作证明了利用基于植物的瞬时表达系统生产负担得起且具有免疫原性的HPV疫苗的潜力，特别是对于发展中国家。
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