Gómez-Barroso Mariana, Moreno-Calderón Koré M, Sánchez-Duarte Elizabeth, Cortés-Rojo Christian, Saavedra-Molina Alfredo, Rodríguez-Orozco Alain R, Montoya-Pérez Rocío
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia, Michoacán 58030, Mexico.
Departamento de Ciencias Aplicadas al Trabajo, Universidad de Guanajuato Campus León, Eugenio Garza Sada 572, Lomas del Campestre Sección 2, León, Guanajuato 37150, Mexico.
Antioxidants (Basel). 2020 Dec 4;9(12):1232. doi: 10.3390/antiox9121232.
Obesity causes insulin resistance and hyperinsulinemia which causes skeletal muscle dysfunction resulting in a decrease in contraction force and a reduced capacity to avoid fatigue, which overall, causes an increase in oxidative stress. K channel openers such as diazoxide and the implementation of exercise protocols have been reported to be actively involved in protecting skeletal muscle against metabolic stress; however, the effects of diazoxide and exercise on muscle contraction and oxidative stress during obesity have not been explored. This study aimed to determine the effect of diazoxide in the contraction of skeletal muscle of obese male Wistar rats (35 mg/kg), and with an exercise protocol (five weeks) and the combination from both. Results showed that the treatment with diazoxide and exercise improved muscular contraction, showing an increase in maximum tension and total tension due to decreased ROS and lipid peroxidation levels and improved glutathione redox state. Therefore, these results suggest that diazoxide and exercise improve muscle function during obesity, possibly through its effects as K channel openers.
肥胖会导致胰岛素抵抗和高胰岛素血症，进而引起骨骼肌功能障碍，导致收缩力下降以及避免疲劳的能力降低，总体上会导致氧化应激增加。据报道，诸如二氮嗪之类的钾通道开放剂以及运动方案的实施积极参与保护骨骼肌免受代谢应激；然而，尚未探究二氮嗪和运动对肥胖期间肌肉收缩和氧化应激的影响。本研究旨在确定二氮嗪对肥胖雄性Wistar大鼠（35毫克/千克）骨骼肌收缩的影响，并研究运动方案（五周）以及二者联合使用的影响。结果表明，用二氮嗪和运动进行治疗可改善肌肉收缩，由于活性氧（ROS）和脂质过氧化水平降低以及谷胱甘肽氧化还原状态改善，最大张力和总张力增加。因此，这些结果表明，二氮嗪和运动可能通过其作为钾通道开放剂的作用改善肥胖期间的肌肉功能。
