Coelho Catarina A, Bordelo JoÃo P, Camassa JosÉ A, Barros Vera A, Babo Pedro S, Gomes Manuela E, Reis Rui L, Azevedo Jorge T DE, Requicha JoÃo F, FaÍsca Pedro, Carvalho Pedro P, Viegas Carlos A, Dias Isabel R
Faculty of Veterinary Medicine (FMV), University Lusófona de Humanidades e Tecnologias (ULHT), Campo Grande, 376 1749-024 Lisbon, Portugal.
Department of Veterinary Sciences, School of Agricultural and Veterinary Sciences (ECAV), University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.
An Acad Bras Cienc. 2020 Dec 7;92(4):e20200435. doi: 10.1590/0001-3765202020200435. eCollection 2020.
Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.
骨质疏松症是一种代谢紊乱疾病，其特征是骨量和骨结构丢失，增加了脆性骨折的风险，多见于绝经后女性。绵羊是公认的用于骨质疏松症研究的大型动物模型。将一组3至4岁的母羊作为实验组，进行卵巢切除术（OVX）并每周应用糖皮质激素（GC），持续24周，并与假手术对照组进行比较。在进行OVX和给予GC之前及之后24周分析血液和骨髓参数。在研究结束时，通过对L4椎骨进行显微计算机断层扫描和组织形态计量分析来确认骨质减少。观察到平均红细胞体积、平均细胞血红蛋白和单核细胞有统计学意义的增加，而红细胞计数和嗜酸性粒细胞减少（p<0.05）。碱性磷酸酶（ALP）、γ-谷氨酰转肽酶、镁和α1球蛋白增加，而肌酐、白蛋白、钠和雌二醇减少（p<0.05）。观察到骨形成标志物（骨ALP和骨钙素）略有下降，骨吸收标志物（I型胶原C末端肽和抗酒石酸酸性磷酸酶）略有增加，但无统计学意义。本研究旨在有助于更好地了解绵羊作为骨质疏松症研究模型以及所实施的诱导方案可能对机体代谢产生的影响。
