Dittmer Keren E, Chernyavtseva Anastasia, Marshall Jonathan C, Cabrera Diana, Wolber Frances M, Kruger Marlena
School of Veterinary Science, Massey University, Palmerston North 4442, New Zealand.
School of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand.
Animals (Basel). 2021 Dec 29;12(1):67. doi: 10.3390/ani12010067.
Osteoporosis is a significant public health issue around the world, with post-menopausal osteoporosis due to estrogen deficiency resulting in approximately ¾ of cases. In this study, 18 aged Merino ewes were ovariectomized, and 10 were controls. Three of the ovariectomized ewes were treated weekly with 400 mg of methylprednisolone for 5 months and three were treated weekly for 2 months, followed by a 3-month recovery period. At 2 months, five control animals and six ovariectomized animals were euthanized. At 5 months, all the remaining ewes were euthanized. Kidney samples were collected postmortem for qPCR analysis of , , , , , , , , , , , , , and Ovariectomized sheep had significantly greater expression compared with other groups. Ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months showed significant differences in , and gene expression compared to other groups. Differences in expression were most marked after adjustment for repeated measures ( = 0.1). is known as the "anti-aging" hormone and is involved in calcium and phosphorus metabolism. may be involved in the recovery of bone mineral density in ovariectomized sheep treated with glucocorticoids for 2 months followed by euthanasia at 5 months. Further research on the role of is recommended.
骨质疏松症是全球一个重大的公共卫生问题，约四分之三的病例是由于雌激素缺乏导致的绝经后骨质疏松症。在本研究中，18只成年美利奴母羊接受了卵巢切除术，10只为对照组。三只接受卵巢切除术的母羊每周接受400毫克甲基强的松龙治疗，持续5个月，三只每周治疗2个月，随后有3个月的恢复期。在2个月时，对5只对照动物和6只接受卵巢切除术的动物实施安乐死。在5个月时，对所有剩余的母羊实施安乐死。死后采集肾脏样本，用于对……进行qPCR分析。与其他组相比，接受卵巢切除术的绵羊……表达明显更高。接受糖皮质激素治疗2个月后在5个月时实施安乐死的接受卵巢切除术的绵羊，与其他组相比，在……和……基因表达上存在显著差异。在对重复测量进行调整后，……表达差异最为明显（=0.1）。……被称为“抗衰老”激素，参与钙和磷的代谢。……可能参与了接受糖皮质激素治疗2个月后在5个月时实施安乐死的接受卵巢切除术的绵羊骨矿物质密度的恢复。建议对……的作用进行进一步研究。
