一个在酿酒酵母的a、α和HO菌株中显示基因组变异的DNA序列。

A DNA sequence which shows genomic variation in a, alpha and HO strains of Saccharomyces cerevisiae.

作者信息

Gupta N J, Jones K W

机构信息

Genetics Department, University of Edinburgh, UK.

出版信息

Curr Genet. 1987;12(3):161-6. doi: 10.1007/BF00436874.

DOI:10.1007/BF00436874

PMID:3329574

Abstract

HindIII digested DNA from various mutant strains of Saccharomyces cerevisiae probed with a 340 bp nucleotide sequence in M13mp8 derived from a mouse liver cDNA clone p1581 showed strong hybridization to a 4.1 kb DNA fragment class. This was limited to the DNA of cells of alpha mating type but the fragments concerned apparently do not originate from chromosome III. The pattern of hybridization was modified in strains carrying the HO gene consistent with there being extra copies of the Bkm-homologous sequence in these cells. Northern analysis of RNA from cells synchronised in various stages of the mitotic and meiotic cell cycle probed with M13mp8/p1581 indicated related transcripts in meiotic cells.

摘要

用源自小鼠肝脏cDNA克隆p1581的M13mp8中的340 bp核苷酸序列探测经HindIII消化的酿酒酵母各种突变菌株的DNA，结果显示与一个4.1 kb的DNA片段类别有强烈杂交。这仅限于α交配型细胞的DNA，但相关片段显然并非源自第三条染色体。在携带HO基因的菌株中，杂交模式发生了改变，这与这些细胞中存在Bkm同源序列的额外拷贝一致。用M13mp8/p1581探测处于有丝分裂和减数分裂细胞周期不同阶段同步化的细胞的RNA的Northern分析表明，减数分裂细胞中有相关转录本。